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Related Experiment Video

Updated: Feb 25, 2026

Characterizing Epithelial Wound Healing In Vivo Using the Cnidarian Model Organism Clytia hemisphaerica
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Membrane wound healing at single cellular level.

Rehana Afrin1, Masakazu Saito1, Takahiro Watanabe-Nakayama1

  • 1Innovation Laboratory, Tokyo Institute of Technology, Midori-ku, Yokohama, Japan.

Nanomedicine : Nanotechnology, Biology, and Medicine
|July 31, 2017
PubMed
Summary
This summary is machine-generated.

We developed a nano-technological method using atomic force microscopy (AFM) to create and observe micrometer-sized holes in live cell membranes, modeling molecular wound healing and resealing processes.

Keywords:
Lipid refilling rateNanotechnologyStress fiber repair processWound creationWound healing

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Area of Science:

  • Cell biology
  • Nanotechnology
  • Biophysics

Background:

  • Cell membrane integrity is crucial for cellular function.
  • Understanding cellular repair mechanisms at the molecular level is essential.
  • Wound healing in cells involves complex membrane dynamics.

Purpose of the Study:

  • To develop a nano-technological method for creating controlled membrane damage.
  • To investigate the resealing process of live cell membranes at the single-cell level.
  • To model molecular-level wound healing using atomic force microscopy (AFM).

Main Methods:

  • Live cell membranes were fluorescently labeled with Kusabira Orange (KO).
  • A glass bead on an AFM cantilever, modified with phospholipase A2, created micrometer-sized holes.
  • The resealing rate and membrane dynamics were monitored using fluorescence microscopy.

Main Results:

  • Micrometer-sized holes (4-14 μm²) were successfully created on cell membranes.
  • Hole resealing occurred at an average rate of ~0.014 μm²/s.
  • Bleb formation accompanied hole creation, and surrounding fluorescent material dispersed over time.

Conclusions:

  • AFM provides a precise tool for studying cell membrane damage and repair.
  • The study successfully models molecular-level wound healing in live cells.
  • The observed resealing dynamics offer insights into membrane repair mechanisms.