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Updated: Feb 25, 2026

Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice
Published on: March 11, 2020
A single spike deteriorates synaptic conductance estimation
Ryota Kobayashi1, Hiroshi Nishimaru2, Hisao Nishijo2
1Principles of Informatics Research Division, National Institute of Informatics, 2-1-2 Hitotsubashi, Chiyoda-ku, Tokyo, Japan; Department of Informatics, Graduate University for Advanced Studies (Sokendai), 2-1-2 Hitotsubashi, Chiyoda-ku, Tokyo, Japan.
Abstract:
We investigated the estimation accuracy of synaptic conductances by analyzing simulated voltage traces generated by a Hodgkin-Huxley type model. We show that even a single spike substantially deteriorates the estimation. We also demonstrate that two approaches, namely, negative current injection and spike removal, can ameliorate this deterioration.
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