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Related Experiment Video

Updated: Jan 18, 2026

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

Phenotyping first-generation genome editing mutants: a new standard?

Lydia Teboul¹, Stephen A Murray², Patrick M Nolan³

¹The Mary Lyon Centre, MRC Harwell Institute, Harwell Science Campus, Didcot, Oxfordshire, UK. l.teboul@har.mrc.ac.uk.

Mammalian Genome : Official Journal of the International Mammalian Genome Society

|July 31, 2017

Summary

CRISPR/Cas9 genome engineering enables rapid mutant founder generation for research. However, these first-generation mutants are often genetic mosaics, posing challenges for scientific validity and data interpretation.

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Area of Science:

Genetics
Molecular Biology
Bioengineering

Background:

The CRISPR/Cas9 system offers high efficiency for genome engineering.
Conventional methods for generating phenotyping cohorts involve multi-generational crosses, which are time-consuming.
CRISPR/Cas9 technology allows for the creation of mutant founders, potentially accelerating research.

Purpose of the Study:

To discuss the benefits of using first-generation CRISPR/Cas9-generated mutant founders.
To outline the challenges associated with genetic mosaicism in these mutants.
To evaluate the scientific validity of employing such models in research.

Main Methods:

Literature review and discussion of CRISPR/Cas9 technology.
Analysis of genetic mosaicism in first-generation mutants.
Evaluation of research models utilizing mutant founders.

Main Results:

First-generation mutants generated by CRISPR/Cas9 can accelerate research by bypassing traditional breeding schemes.
These mutants frequently exhibit genetic mosaicism, leading to a complex and undefined genetic makeup.
The presence of mosaicism raises questions about the scientific validity and reproducibility of studies using these models.

Conclusions:

Mutant founders generated via CRISPR/Cas9 offer a promising avenue for accelerated research.
Addressing the challenges of genetic mosaicism is crucial for ensuring the reliability of these models.
Further investigation into the scientific validity of mosaic mutant founders is warranted.