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iPS Cell Differentiation

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.

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Related Experiment Video

Updated: Feb 25, 2026

Generation and Characterization of Human Induced Pluripotent Stem Cell-derived Astrocytes Lacking Fragile X Messenger Ribonucleoprotein

Generation and Characterization of Human Induced Pluripotent Stem Cell-derived Astrocytes Lacking Fragile X Messenger Ribonucleoprotein

Published on: June 6, 2025

An Efficient Platform for Astrocyte Differentiation from Human Induced Pluripotent Stem Cells.

Julia Tcw¹, Minghui Wang², Anna A Pimenova¹

¹Department of Neuroscience & Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, 1425 Madison Avenue, New York, NY 10029, USA; Ronald M. Loeb Center for Alzheimer's disease, Icahn School of Medicine at Mount Sinai, 1425 Madison Avenue, New York, NY 10029, USA.

Stem Cell Reports

|August 1, 2017

Summary

Researchers developed a fast method to create pure human astrocytes from stem cells. These astrocytes mimic natural cells and can be used to study neurological and psychiatric disorders.

Keywords:

astrocyte human induced pluripotent stem cell iPSC

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Synaptic Microcircuit Modeling with 3D Cocultures of Astrocytes and Neurons from Human Pluripotent Stem Cells

Synaptic Microcircuit Modeling with 3D Cocultures of Astrocytes and Neurons from Human Pluripotent Stem Cells

Published on: August 16, 2018

Area of Science:

Neuroscience
Stem Cell Biology
Astrocyte Biology

Background:

Glia, especially astrocytes, play a crucial role in neurological and psychiatric diseases.
Existing methods for generating astrocytes from human induced pluripotent stem cells (hiPSCs) can be complex and time-consuming.

Purpose of the Study:

To develop a rapid, robust, and reproducible protocol for differentiating pure populations of replicative astrocytes from hiPSCs.
To characterize the generated hiPSC-astrocytes and assess their suitability for disease modeling.

Main Methods:

Differentiation of hiPSCs into neural progenitor cells (NPCs) followed by astrocyte differentiation using a single medium.
Evaluation of the protocol across 42 NPC lines from 30 individuals.
Transcriptomic analysis, assessment of response to inflammatory stimuli, phagocytic capacity, and calcium transient activity.

Main Results:

The protocol rapidly (<30 days) generates highly pure hiPSC-astrocytes.
Transcriptomic analysis shows hiPSC-astrocytes resemble primary human fetal astrocytes and are in a non-reactive state.
hiPSC-astrocytes exhibit functional characteristics including response to inflammation, phagocytosis, enhancement of microglial phagocytosis, and spontaneous calcium activity.

Conclusions:

This straightforward and rapid protocol provides a reliable source of hiPSC-astrocytes for research.
These hiPSC-astrocytes are suitable for co-culture studies (neuron-astrocyte and microglia-astrocyte) to investigate neuropsychiatric disorders.
The method offers a valuable tool for advancing the understanding and treatment of brain diseases.