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Pepsin in the toad Bufo marinus.

P M Taylor, M J Tyler

    Comparative Biochemistry and Physiology. A, Comparative Physiology
    |January 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    This study biochemically analyzed pepsinogen in the toad Bufo marinus, finding its optimal pH is 1.6. Lower temperatures reduced pepsinogen activity, offering insights into gastric brooding mechanisms in Rheobatrachus frogs.

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    Area of Science:

    • Comparative physiology
    • Biochemistry
    • Gastrointestinal physiology

    Background:

    • The anuran upper gastrointestinal tract harbors pepsinogen, a precursor to pepsin.
    • Understanding pepsinogen's properties is crucial for digestive processes in amphibians.
    • Gastric brooding in Rheobatrachus frogs presents unique physiological challenges.

    Purpose of the Study:

    • To determine the macroscopic localization of pepsinogen in Bufo marinus.
    • To ascertain the pH optimum for anuran pepsin activity.
    • To investigate the impact of temperature on pepsinogen and its activity.

    Main Methods:

    • Biochemical assays were employed to localize pepsinogen.
    • The pH optimum of pepsin was determined through enzyme activity measurements.

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  • Stored pepsinogen and proteolytic activity were assessed at lowered ambient temperatures.

    • Main Results:

    • Pepsinogen was localized to the upper gastrointestinal tract of Bufo marinus.
    • The determined pH optimum for anuran pepsin was 1.6.
    • Reduced ambient temperature led to decreased pepsinogen levels and proteolytic activity.

    Conclusions:

    • The findings provide biochemical data on anuran pepsinogen and pepsin.
    • Temperature sensitivity of pepsinogen suggests physiological constraints.
    • Results offer a basis for speculating on gastric brooding mechanisms in Rheobatrachus.