Related Experiment Video
Updated: Feb 17, 2026

Author Spotlight: Investigating Fungal Pathogenicity Mechanisms in Maize
Published on: September 15, 2023
WRKY45 phosphorylation at threonine 266 acts negatively on WRKY45-dependent blast resistance in rice
Yoshihisa Ueno1,2, Akane Matsushita1, Haruhiko Inoue1
1a Disease Resistant Crops Research Unit , National Institute of Agrobiological Sciences , Tsukuba , Ibaraki , Japan.
Abstract:
WRKY45 is a central regulator of disease resistance mediated by salicylic acid signaling in rice and its activation involves phosphorylation by OsMPK6. OsMPK6 phosphorylates WRKY45 at Thr266, Ser294, and Ser299 in vitro. Phosphorylation of Ser294 and/or Ser299 is required for full activation of WRKY45, but the importance of Thr266 phosphorylation has remained unknown. Here, we report on the characterization of Thr266 phosphorylation of WRKY45 in rice. Transient expression of mutant WRKY45 revealed that Thr266 is phosphorylated in vivo, together with Ser294/299. Replacement of Thr266 by Asn did not affect the enhanced Magnaporthe oryzae resistance afforded by WRKY45 overexpression. By contrast, replacement by Asp negated the enhancement of M. oryzae resistance. These results suggest that Thr266 phosphorylation acts negatively on WRKY45-dependent disease resistance.
More Related Videos
09:25Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae
Published on: June 2, 2021
07:14The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea
Published on: August 4, 2018
Related Concept Videos
Transcriptional Regulation: Riboswitches
Cell Signaling in Plants
Riboswitches
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...