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Highly resolved HSQC experiments for the fast and accurate measurement of homonuclear and heteronuclear coupling
Alexandre A Souza1, Roberto R Gil2, Teodor Parella3
1Servei de Ressonància Magnètica Nuclear, Universitat Autònoma de Barcelona, E-08193 Bellaterra (Barcelona), Catalonia, Spain; Departamento de Química, Universidade Federal do Piauí, 64049-550 Teresina, PI, Brazil.
Abstract:
A number of J-upscaled NMR experiments are currently available to measure coupling constants along the indirect F1 dimension of a 2D spectrum. A major drawback is the limited F1 digital resolution that requires long acquisition times in order to achieve reasonably accurate measures. Here is shown how high levels of F1 digital resolution in a multiple-purpose HSQC experiment can be easily achieved by implementing a general J/δ-scaling strategy. In particular, a set of new J-resolved HSQC experiments is presented for a faster and much more accurate J determination in small molecules. Several options and practical aspects are discussed and exemplified by measuring the magnitude and/or the sign of several homo- and heteronuclear coupling constants in one shot.