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Related Experiment Video

Updated: Feb 25, 2026

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Sustained and Cost Effective Silver Substrate for Surface Enhanced Raman Spectroscopy Based Biosensing.

Jian Ju1, Wei Liu1, Clint Michael Perlaki1

  • 1School of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, Singapore, 637457, Singapore.

Scientific Reports
|August 2, 2017
PubMed
Summary

We developed a novel silver nanoparticle substrate protected by nitrogen-doped Graphene Quantum Dots (Ag NP@N-GQD) for enhanced and sustained surface-enhanced Raman spectroscopy (SERS) biosensing. This cost-effective substrate shows improved stability for field applications.

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Area of Science:

  • Nanomaterials Science
  • Analytical Chemistry
  • Biosensing Technology

Background:

  • Surface-enhanced Raman spectroscopy (SERS) shows promise for point-of-care diagnostics.
  • Current silver-based SERS substrates have limited stability for field use.
  • Developing robust and cost-effective SERS substrates is crucial for practical biosensing.

Purpose of the Study:

  • To develop a novel, sustained, and cost-effective SERS substrate for field biosensing.
  • To evaluate the performance of the new substrate for glucose detection.
  • To enhance the stability and sensitivity of SERS active materials.

Main Methods:

  • Synthesis of silver nanoparticles (Ag NPs) protected by nitrogen-doped Graphene Quantum Dots (N-GQDs) forming Ag NP@N-GQD.
  • Systematic evaluation of the Ag NP@N-GQD substrate for SERS.
  • Assessment of substrate stability under various environmental conditions (wet and dry).
  • Application of the substrate for glucose detection in mouse blood samples.

Main Results:

  • The Ag NP@N-GQD substrate exhibited significantly stronger Raman enhancement than pure Ag NPs.
  • The Ag NP@N-GQD substrate maintained SERS performance for at least 30 days, compared to 10 days for pure Ag NPs.
  • Successful application of the dry Ag NP@N-GQD thin film for glucose detection in mouse blood.

Conclusions:

  • Ag NP@N-GQD is a cost-effective and sustained SERS substrate with enhanced performance.
  • The developed substrate overcomes the limitations of traditional SERS substrates for field applications.
  • This advancement is a significant step towards practical SERS-based field biosensing.