Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

3.0K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
3.0K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

925
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
925

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Distinguishing Ki67 stratification expression in ER-positive/HER2-negative breast cancers: comparison of advanced MRI diffusion models.

BMC medical imaging·2026
Same author

Anti-metastatic effect of Paris polyphylla var. yunnanensis and Paris fargesii in esophageal cancer preclinical models.

Journal of ethnopharmacology·2026
Same author

The Regional Vulnerability Index (RVI) as a Neuroimaging-Based Biomarker for Autism: Associations with Likelihood, Cognition, and Longitudinal Social Outcomes.

bioRxiv : the preprint server for biology·2026
Same author

Brainwide Structural Markers of Familial and Clinical Risk for Bipolar Disorder in Youth.

Biological psychiatry. Cognitive neuroscience and neuroimaging·2026
Same author

<i>Andrographis paniculata</i> Inhibits Tongue Squamous Cell Carcinoma via Regulating Wnt/β-Catenin Signaling and Epithelial-Mesenchymal Transition.

International journal of molecular sciences·2026
Same author

Detective quantum efficiency of the Timepix4 hybrid pixel detector and its application to parallel-beam diffraction.

Ultramicroscopy·2026
Same journal

Chlorinated VSLSs Surpass HCFCs in CFC-11-Equivalent Emissions for Ozone Layer Depletion in China.

Nature communications·2026
Same journal

Author Correction: Charge transfer in triphenylamine-tetrazine covalent organic frameworks for solar-driven hydrogen peroxide production.

Nature communications·2026
Same journal

Vegetation browning patterns under compound soil and atmospheric dryness in northern permafrost ecosystems.

Nature communications·2026
Same journal

Voltage imaging of CA1 pyramidal cells and SST+ interneurons reveals stability and plasticity mechanisms of spatial firing.

Nature communications·2026
Same journal

Radical-omics reveals the hydrogen-abstraction pathway of isoprene oxidation.

Nature communications·2026
Same journal

Toughening elastomer via sequentially activated multi-pathway energy dissipation.

Nature communications·2026
See all related articles

Related Experiment Video

Updated: Feb 25, 2026

Three-dimensional Characterization of Interorganelle Contact Sites in Hepatocytes using Serial Section Electron Microscopy
09:12

Three-dimensional Characterization of Interorganelle Contact Sites in Hepatocytes using Serial Section Electron Microscopy

Published on: June 9, 2022

6.4K

Electron ptychographic microscopy for three-dimensional imaging.

Si Gao1, Peng Wang2,3, Fucai Zhang4,5,6

  • 1National Laboratory of Solid State Microstructures, College of Engineering and Applied Sciences, Collaborative Innovation Center of Advanced Microstructures and Center for the Microstructures of Quantum Materials, Nanjing University, Nanjing, 210093, People's Republic of China.

Nature Communications
|August 2, 2017
PubMed
Summary
This summary is machine-generated.

Researchers developed a new 3D imaging technique combining electron ptychography and inverse multislice methods. This approach achieves 0.34 nm lateral resolution for nanostructured materials, advancing nanoscale structural determination.

More Related Videos

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization
06:33

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Published on: October 29, 2019

10.8K
Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation
12:59

Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation

Published on: February 28, 2021

4.2K

Related Experiment Videos

Last Updated: Feb 25, 2026

Three-dimensional Characterization of Interorganelle Contact Sites in Hepatocytes using Serial Section Electron Microscopy
09:12

Three-dimensional Characterization of Interorganelle Contact Sites in Hepatocytes using Serial Section Electron Microscopy

Published on: June 9, 2022

6.4K
Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization
06:33

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Published on: October 29, 2019

10.8K
Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation
12:59

Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation

Published on: February 28, 2021

4.2K

Area of Science:

  • Materials Science
  • Nanotechnology
  • Electron Microscopy

Background:

  • Understanding nanoscale material properties requires 3D structural information.
  • Electron tomography is a common method for 3D imaging but has limitations.
  • 3D electron ptychography is challenging due to electron scattering.

Purpose of the Study:

  • To present an alternative method for 3D nanostructure imaging.
  • To demonstrate high-resolution depth sectioning of materials.
  • To enable precise 3D structure determination of nanoscale objects.

Main Methods:

  • Combining electron ptychography with the inverse multislice method.
  • Adapting multi-slice methods for electrons in the multiple scattering regime.
  • Acquiring tilt series of 2D images for 3D reconstruction.

Main Results:

  • Achieved 0.34 nm lateral resolution in depth-sectioned slices.
  • Demonstrated a depth resolution of approximately 24-30 nm.
  • Successfully imaged nanostructured materials in three dimensions.

Conclusions:

  • The developed method offers a powerful alternative for 3D nanoscale imaging.
  • Potential applications include inorganic nanostructures and biological macromolecules.
  • Advances 3D structure determination capabilities using electrons at the nanoscale.