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Concentrations of MUC16 and MUC5AC using three tear collection methods
Anna F Ablamowicz1, Jason J Nichols1
1School of Optometry, Department of Optometry and Vision Science, University of Alabama at Birmingham, Birmingham, AL.
Molecular Vision
|August 2, 2017
Summary
The optimal tear collection method depends on the specific mucin being analyzed. Schirmer's strip collection is best for MUC5AC, while basal tear collection is optimal for MUC16 analysis.
Area of Science:
- Ophthalmology
- Biochemistry
- Proteomics
Background:
- Ocular surface mucins, MUC5AC and MUC16, play crucial roles in maintaining ocular surface health.
- Accurate quantification of these mucins is essential for diagnosing and managing ocular surface diseases.
- Standardized tear collection methods are needed for reliable mucin analysis.
Purpose of the Study:
- To compare the efficacy of three different tear collection methods for analyzing MUC5AC and MUC16.
- To determine the optimal method for collecting tears to maximize MUC5AC and MUC16 detection.
Main Methods:
- Tears were collected from 15 healthy subjects using Schirmer's strip, basal tear collection, and flush tear collection methods.
- MUC5AC and MUC16 concentrations were analyzed using Western blot after protein extraction.
- Statistical analysis, including Kruskal-Wallis and Dunn's multiple comparisons tests, was performed to compare methods.
Main Results:
- Schirmer's strip collection yielded the highest MUC5AC concentration (median signal intensity 2.86).
- Basal tear collection yielded the highest MUC16 concentration (median signal intensity 5.24).
- Statistically significant differences were observed between collection methods for both mucins (p < 0.01).
Conclusions:
- Both MUC5AC and MUC16 are detectable in human tears using various collection methods.
- The choice of tear collection method should be tailored to the specific mucin of interest.
- Schirmer's strip is optimal for MUC5AC, and basal tear collection is optimal for MUC16 analysis.
