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MiR-1298 affects cell proliferation and apoptosis in C6 cells by targeting SET domain containing 7

Chun-Mei Wang1, Bao-Hua Cheng1, Qing-Jie Xue1

  • 1Neurobiology Institute, Jining Medical University, Jining, P.R. China.

International Journal of Immunopathology and Pharmacology
|August 2, 2017
PubMed

Insights

Rattumor cells show decreased levels of microRNA-1298 (miR-1298). Restoring miR-1298 inhibits tumor cell growth and promotes cell death by targeting SET domain containing 7 (SETD7).

Area of Science:

  • Molecular Biology
  • Oncology
  • Neuroscience

Background:

  • MicroRNAs (miRNAs) play crucial roles in cellular processes, including tumor development.
  • Previous studies indicated down-regulation of rno-miR-1298 in a rat ischemia-reperfusion model.
  • The specific function and molecular mechanisms of rno-miR-1298 in rat tumor cells remain largely unexplored.

Purpose of the Study:

  • To investigate the role of rno-miR-1298 in rat C6 glioma cells.
  • To identify the molecular targets and mechanisms underlying rno-miR-1298's function in tumor progression.

Main Methods:

  • Quantitative analysis of rno-miR-1298 expression in C6 cells.
  • Overexpression and knockdown experiments to assess functional impacts.
  • Bioinformatics analysis and luciferase reporter assays to identify miRNA targets.
  • Western blot analysis to confirm target protein regulation.

Main Results:

  • Rno-miR-1298 was significantly down-regulated in rat C6 tumor cells.
  • Overexpression of rno-miR-1298 inhibited C6 cell proliferation and induced apoptosis.
  • SET domain containing 7 (SETD7) was validated as a direct target of rno-miR-1298.
  • Knockdown of SETD7 mimicked the effects of rno-miR-1298 overexpression, suppressing proliferation and promoting apoptosis.

Conclusions:

  • Rno-miR-1298 suppresses proliferation and induces apoptosis in rat glioma cells by targeting SETD7.
  • The miR-1298/SETD7 axis represents a novel regulatory pathway in tumor development.
  • This pathway offers potential therapeutic targets for nervous system tumors.
Keywords:
SET domain containing 7apoptosismiR-1298proliferationtumor cell

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