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Identification of messenger RNA for glutamate dehydrogenase using a spectrophotometric probe

M Hillar, S Orudiakomo

Molecular Biology Reports

|January 1, 1986

Abstract:

Specific messenger RNA for glutamate dehydrogenase was partially purified from a calf liver polysomal poly(A)-mRNA fraction by sucrose density gradient centrifugation. Enzyme activity in the translational incubation mixture was detected by measuring NADH oxidation in the presence of alpha-ketoglutarate and ammonia as a decrease in absorbency 340-442 nm in a dual wavelength Aminco DW-2 spectrophotometer.

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