Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.7K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.7K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

13.5K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
13.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Two-Peak Heat Capacity Accounts for Rln(2) Entropy and Ground State Access in the Dipole-Octupole Pyrochlore Ce_{2}Hf_{2}O_{7}.

Physical review letters·2025
Same author

Corrigendum to "Ultra-high dose rate dosimetry for pre-clinical experiments with mm-small proton fields" [Phys. Med. 104 (2022) 101-111].

Physica medica : PM : an international journal devoted to the applications of physics to medicine and biology : official journal of the Italian Association of Biomedical Physics (AIFB)·2025
Same author

Lensless magneto-optical imaging.

Scientific reports·2025
Same author

Ionization potentials of metal clusters studied with a broad range, tunable vacuum ultraviolet light source.

The Review of scientific instruments·2023
Same author

Characterization of LiF:Mg,Ti thermoluminescence detectors in low-LET proton beams at ultra-high dose rates.

Physics in medicine and biology·2023
Same author

Ultra-high dose rate dosimetry for pre-clinical experiments with mm-small proton fields.

Physica medica : PM : an international journal devoted to the applications of physics to medicine and biology : official journal of the Italian Association of Biomedical Physics (AIFB)·2022

Related Experiment Video

Updated: Feb 25, 2026

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
15:04

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Published on: May 18, 2011

13.6K

Selective sensitivity in Kerr microscopy.

I V Soldatov1, R Schäfer2

  • 1Institute for Metallic Materials, Leibniz Institute for Solid State and Materials Research (IFW) Dresden, Helmholtzstrasse 20, D-01069 Dresden, Germany and Institute of Natural Sciences, Ural Federal University, 620002 Ekaterinburg, Russia.

The Review of Scientific Instruments
|August 3, 2017
PubMed
Summary

A novel magneto-optical Kerr microscopy technique improves contrast separation using synchronized LED illumination. This method enhances sensitivity and eliminates parasitic signals, enabling clearer domain imaging in magnetic materials.

More Related Videos

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.4K
Assessing Protein Interactions in Live-Cells with FRET-Sensitized Emission
09:15

Assessing Protein Interactions in Live-Cells with FRET-Sensitized Emission

Published on: April 22, 2021

4.0K

Related Experiment Videos

Last Updated: Feb 25, 2026

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
15:04

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Published on: May 18, 2011

13.6K
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.4K
Assessing Protein Interactions in Live-Cells with FRET-Sensitized Emission
09:15

Assessing Protein Interactions in Live-Cells with FRET-Sensitized Emission

Published on: April 22, 2021

4.0K

Area of Science:

  • Materials Science
  • Condensed Matter Physics
  • Optics

Background:

  • Magneto-optical Kerr microscopy (MOKE) is crucial for studying magnetic domain structures.
  • Conventional MOKE setups can suffer from parasitic contrast, limiting sensitivity and image clarity.
  • Effective separation of magnetic contrast is essential for accurate material characterization.

Purpose of the Study:

  • To introduce a new technique for contrast separation in wide-field MOKE.
  • To demonstrate simultaneous real-time display of orthogonal in-plane sensitive domain images.
  • To enhance the sensitivity and eliminate parasitic contrast in MOKE.

Main Methods:

  • Utilizing eight light-emitting diodes (LEDs) for illumination.
  • Synchronously switching LED light with camera exposure.
  • Guiding light to the microscope via glass fibers.

Main Results:

  • Simultaneous display of domain images with orthogonal in-plane sensitivity.
  • Obtained images with pure in-plane or polar contrast.
  • Demonstrated enhanced sensitivity with Kerr rotations as low as 0.6 millidegrees.
  • Achieved a doubling of in-plane domain contrast for Permalloy films, NdFeB magnets, and cobalt crystals.

Conclusions:

  • The new contrast separation technique significantly enhances MOKE sensitivity.
  • Parasitic contrast contributions are effectively eliminated, leading to purer magnetic signals.
  • The technique provides a powerful tool for advanced magnetic domain imaging and material analysis.