Related Experiment Video

Updated: Feb 25, 2026

Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane
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Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane

Published on: February 8, 2020

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5'-tRNA Halves are Dysregulated in Clear Cell Renal Cell Carcinoma

Chenming Zhao1, Yuri Tolkach1, Doris Schmidt1

  • 1Departments of Urology and Pathology (YT, GK), University Hospital Bonn, Bonn, Germany.

The Journal of Urology
|August 3, 2017
PubMed
Abstract

Insights

Four types of 5'-tRNA halves were found to be downregulated in clear cell renal cell carcinoma tissues and serum. These fragments may serve as diagnostic and prognostic biomarkers for kidney cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • RNA fragments, specifically tRNA halves, are dysregulated in various malignancies.
  • Clear cell renal cell carcinoma (ccRCC) is the most common subtype of kidney cancer.

Purpose of the Study:

  • To investigate the expression levels of four specific 5 RNA halves in ccRCC tissues and serum.
  • To evaluate the potential of these 5 RNA halves as diagnostic and prognostic biomarkers for ccRCC.

Main Methods:

  • Prospective collection of tissue and serum samples from ccRCC patients and non-malignant controls.
  • Isolation of total RNA and specific quantification of 5 RNA-Arg-CCT, 5 RNA-Glu-CTC, 5 RNA-Leu-CAG, and 5 RNA-Lys-TTT using TaqMan®-based qRT-PCR.
  • Analysis of expression levels in relation to clinicopathological parameters and disease status.

Main Results:

  • All four studied 5 RNA halves were significantly downregulated in ccRCC tissues compared to normal renal tissues.
  • Decreased expression of 5 RNA halves correlated with adverse clinicopathological parameters, including nonorgan-confined disease and higher ISUP grade (for 5 RNA-Lys-TTT).
  • Lower serum levels of 5 RNA-Arg-CCT, 5 RNA-Glu-CTC, and 5 RNA-Lys-TTT were observed in ccRCC patients compared to controls, suggesting potential as noninvasive biomarkers.

Conclusions:

  • The studied 5 RNA halves demonstrate potential as diagnostic and prognostic biomarkers for ccRCC.
  • The downregulation of 5 RNA halves in ccRCC suggests a possible tumor-suppressive role that warrants further investigation.
Keywords:
RNAbiomarkerscarcinomadiagnosisprognosisrenal celltransfertumor

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