Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prevalence, risk factors, and psychosocial impact of self-reported recurrent oral ulceration among Palestinian medical and dental students: a multicenter cross-sectional study.

BMC oral health·2026
Same author

DDT exposure induces microglial activation and disease-associated microglial signatures: Relevance to mechanisms of Alzheimer's disease.

Neurotoxicology·2025
Same author

Enhanced Cellular Uptake of Compact Cas Proteins: A Comparative Study of Cas12f and Cas9 in Human Cells.

Engineering in life sciences·2025
Same author

Delayed perinephric hematoma following ESWL in an anticoagulated patient: A case report.

International journal of surgery case reports·2025
Same author

Effects of Pesticide Exposure on Neuroinflammation and Microglial Gene Expression: Relevance to Mechanisms of Alzheimer's Disease Risk.

bioRxiv : the preprint server for biology·2025
Same author

Behçet's disease and factor V Leiden: A thrombogenic synergy causing budd-chiari syndrome.

Radiology case reports·2024

Related Experiment Video

Updated: Feb 25, 2026

In Vitro Aggregation Assays Using Hyperphosphorylated Tau Protein
09:22

In Vitro Aggregation Assays Using Hyperphosphorylated Tau Protein

Published on: January 2, 2015

19.1K

Lead exposure and tau hyperphosphorylation: An in vitro study.

Syed Waseem Bihaqi1, Aseel Eid2, Nasser H Zawia3

  • 1George and Anne Ryan Institute for Neuroscience, University of Rhode Island, Kingston, RI, USA.

Neurotoxicology
|August 3, 2017
PubMed
Summary

Lead exposure alters tau protein and promotes its hyperphosphorylation in neuronal cells, suggesting environmental factors influence neurodegenerative diseases like Alzheimer's disease.

Keywords:
CDK5Hyperphosphorylated tauLead (Pb)SH-SY5YTau proteinp35/p25

More Related Videos

An In Vitro Model for Studying Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons
05:51

An In Vitro Model for Studying Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons

Published on: May 23, 2019

6.5K
Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells
12:55

Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells

Published on: October 10, 2017

9.5K

Related Experiment Videos

Last Updated: Feb 25, 2026

In Vitro Aggregation Assays Using Hyperphosphorylated Tau Protein
09:22

In Vitro Aggregation Assays Using Hyperphosphorylated Tau Protein

Published on: January 2, 2015

19.1K
An In Vitro Model for Studying Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons
05:51

An In Vitro Model for Studying Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons

Published on: May 23, 2019

6.5K
Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells
12:55

Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells

Published on: October 10, 2017

9.5K

Area of Science:

  • Neuroscience
  • Toxicology
  • Cell Biology

Background:

  • Fibrillary lesions, primarily tau protein (MAPT), are hallmarks of neurodegenerative diseases like Alzheimer's disease (AD).
  • Tau dysregulation, including hyperphosphorylation, leads to neuronal dysfunction and death.
  • Previous research linked lead (Pb) exposure to increased tau phosphorylation in animal models.

Purpose of the Study:

  • To investigate the effects of lead (Pb) exposure on tau protein and its phosphorylation in human neuroblastoma cells.
  • To determine the role of Pb in altering tau levels, site-specific hyperphosphorylation, and associated signaling pathways (CDK5/p35/p25).

Main Methods:

  • Differentiated human neuroblastoma SH-SY5Y cells were exposed to varying concentrations of lead (5-100μM) for 48 hours.
  • Western blot analysis was used to assess total tau, site-specific tau hyperphosphorylation, cyclin-dependent kinase 5 (CDK5), and p35/p25 protein levels at different time points post-exposure (24-144h).

Main Results:

  • Lead exposure resulted in aberrant tau levels and site-specific tau hyperphosphorylation in SH-SY5Y cells.
  • Elevated CDK5 levels and altered p35/p25 protein ratios were observed, particularly at 72 and 144 hours after lead exposure cessation.
  • These molecular changes indicate lead-induced neurotoxic effects on tau pathology.

Conclusions:

  • Lead exposure induces tau alterations and hyperphosphorylation in neuronal cells.
  • Environmental factors like lead can influence the molecular mechanisms underlying neurodegeneration.
  • Findings support the link between environmental exposures and the development of neurodegenerative events.