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Related Experiment Video

Updated: Feb 25, 2026

Determination of the Mating Efficiency of Haploids in Saccharomyces cerevisiae

Determination of the Mating Efficiency of Haploids in Saccharomyces cerevisiae

Published on: December 2, 2022

Mating-Type Determination in Schizosaccharomyces pombe.

Karl Ekwall¹, Geneviève Thon²

¹Department of Biosciences and Nutrition, Karolinska Institute, Stockholm SE-141 83, Sweden; karl.ekwall@ki.se.

Cold Spring Harbor Protocols

|August 3, 2017

Summary

This study details two methods for determining mating types in fission yeast, Schizosaccharomyces pombe. These include traditional mating assays and modern polymerase chain reaction techniques for mat1 gene analysis.

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Last Updated: Feb 25, 2026

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Area of Science:

* Molecular Biology
* Genetics
* Mycology

Background:

* Understanding mating-type determination is crucial for genetic studies in fission yeast.
* Schizosaccharomyces pombe is a key model organism in biological research.

Purpose of the Study:

* To outline established protocols for mating-type testing in Schizosaccharomyces pombe.
* To present complementary molecular methods for mating-type analysis.

Main Methods:

* Classical mating assays using specific 'h' and 'h' tester strains.
* Molecular analysis via polymerase chain reaction (PCR) to detect mat1 gene content.

Main Results:

* Demonstration of reliable mating-type determination through both phenotypic (mating) and genotypic (PCR) approaches.
* Validation of PCR as an efficient method for assessing mat1 alleles.

Conclusions:

* Both mating assays and PCR provide robust methods for mating-type determination in Schizosaccharomyces pombe.
* These techniques are essential for researchers working with this fission yeast model system.