Proteogenomics data for deciphering Frankia coriariae interactions with root exudates from three host plants

Area of Science:

• Microbiology
• Plant-Microbe Interactions
• Proteomics

Background:

• Frankia bacteria form symbiotic relationships with actinorhizal plants.
• Understanding Frankia's response to host plant cues is crucial for symbiosis research.
• Root exudates play a significant role in mediating plant-microbe communication.

Purpose of the Study:

• To investigate the proteomic changes in Frankia coriariae BMG5.1 upon exposure to root exudates from compatible, incompatible, and non-host plants.
• To identify bacterial proteins and exoproteins modulated by host plant interactions.
• To provide insights into the molecular mechanisms governing Frankia-plant symbiosis.

Main Methods:

• Incubation of Frankia coriariae BMG5.1 with root exudates from Coriaria myrtifolia (compatible), Alnus glutinosa (incompatible), and Cucumis melo (non-actinorhizal).
• High-throughput proteomics analysis of bacterial cells and exoproteomes using Q-Exactive HF mass spectrometry.
• MS/MS spectra assignment using protein sequence databases from Frankia strains BMG5.1 and Dg1.

Main Results:

• Differential protein expression was observed in Frankia cells and exoproteomes based on the source of root exudates.
• Specific proteins and exoproteins were identified as being modulated by host plant interactions.
• The study provides a comprehensive proteomic dataset of Frankia responses to plant cues.

Conclusions:

• Frankia coriariae exhibits distinct proteomic profiles in response to root exudates from different plant types.
• These findings contribute to understanding the molecular basis of host recognition and symbiosis establishment in Frankia.
• The deposited proteomic data serve as a valuable resource for future research in Frankia symbiosis.