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Updated: Feb 25, 2026

A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl- Channels and Transporters
Published on: April 20, 2015
Dissecting the chloride-nitrate anion transport assay
Yufeng Yang1, Xin Wu2, Nathalie Busschaert3
1Chemistry, University of Southampton, Southampton, SO17 1BJ, UK and Department of Chemistry and Biochemistry, Kyushu University, Fukuoka, 819-0395, Japan.
Abstract:
A systematic study of chloride vs. nitrate selectivity across six anion transporters has revealed a good correlation between the selectivities of their anion binding and membrane transport properties. This work reveals the limitations of the chloride-nitrate exchange assay and shows how new approaches can be used to measure anion uniport.
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