Basic Fibroblast Growth Factor Improved Angiogenesis of Vitrified Human Ovarian Tissues after in vitro Culture and
1Reproductive Medical Center of West China 2nd University Hospital, Sichuan University, Chengdu, Sichuan, China.
Background:
Basic fibroblast growth factor is a potent angiogenic factor.
Objective:
To study the concentration and in vitro culture time of bFGF that maximize the angiogenesis for transplanted human ovarian tissues.
Materials And Methods:
Vitrified and rewarmed human ovarian tissues were cultured in vitro with bFGF (0, 25, 50, 100, 150 and 200 ng/ml) for different periods (1 h, 2 d, 5 d and 7 d) before transplantation. The effect of bFGF on follicle survival was studied by evaluating the pregraft group, control group (no bFGF) and bFGF-treated group. CD34, Ki-67 and AC-3 immuno-histochemical (IHC) staining and histological analysis was used to evaluate angiogenesis, proliferration, apoptosis and follicular quantity.
Results:
Treatments with 100 and 150 ng/ml bFGF improved the angiogenesis for grafted human ovarian tissues after in vitro culture for 2 days. The proliferation and survival of follicles were significantly increased.
Conclusion:
bFGF improved the quality of vitrified-warmed human ovarian tissues after transplantation.
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