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Updated: Feb 25, 2026

Protocol for Dengue Infections in Mosquitoes A. aegypti and Infection Phenotype Determination
Published on: July 4, 2007
Complete coding sequence of dengue virus serotype 4 isolated from field-caught mosquitoes in Thailand
Thikhumporn Sittivicharpinyo1, Passorn Wonnapinij1,2, Wunrada Surat1,2
1Kasetsart University, Faculty of Science, Department of Genetics, Evolutionary Genetics and Computational Biology Research Unit, Bangkok, Thailand.
Abstract:
This report is the first to characterise the complete coding sequence of a dengue virus serotype 4 (DENV-4) genotype I that was isolated from field-caught mosquitoes from an endemic area in Thailand in June 2013. The sequence was assembled from high-throughput sequencing reads generated by Illumina HiSeq. Three out of four observed intra-sample variants caused an amino acid variation in C, NS2B, and NS5 genes. The C4279T variant located in the NS2B gene can indirectly affect the proteolytic activity of the NS3 protein. The sequence provided in this study might be useful for the epidemiological study of DENV-4.

