Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The rhesus monkey: a primate model for hemopoietic stem cell studies.

R L Monroy, T J MacVittie, J H Darden

    Experimental Hematology
    |November 1, 1986
    PubMed
    Summary

    Rhesus monkey bone marrow contains distinct cell populations. Counterflow centrifugation-elutriation separated lymphocyte-rich cells (CP 1-7) and stem cell-rich cells (CP 8-10), impacting lymphohemopoietic reconstitution.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Organ Doses Associated with Partial-Body Irradiation with 2.5% Bone Marrow Sparing of the Non-Human Primate: A Retrospective Study.

    Radiation research·2017
    Same author

    A randomized, open-label, multicenter, phase II study evaluating the efficacy and safety of BTH1677 (1,3-1,6 beta glucan; Imprime PGG) in combination with cetuximab and chemotherapy in patients with advanced non-small cell lung cancer.

    Investigational new drugs·2017
    Same author

    Two randomized, double-blind, placebo-controlled, dose-escalation phase 1 studies evaluating BTH1677, a 1, 3-1,6 beta glucan pathogen associated molecular pattern, in healthy volunteer subjects.

    Investigational new drugs·2016
    Same author

    Filgrastim for the treatment of hematopoietic acute radiation syndrome.

    Drugs of today (Barcelona, Spain : 1998)·2015
    Same author

    Pegfilgrastim administered in an abbreviated schedule, significantly improved neutrophil recovery after high-dose radiation-induced myelosuppression in rhesus macaques.

    Radiation research·2012
    Same author

    ICRP publication 118: ICRP statement on tissue reactions and early and late effects of radiation in normal tissues and organs--threshold doses for tissue reactions in a radiation protection context.

    Annals of the ICRP·2012

    Area of Science:

    • Hematology
    • Cell Biology
    • Immunology

    Background:

    • Bone marrow contains heterogeneous cell populations crucial for lymphohemopoietic reconstitution.
    • Hemopoietic stem cells (HSC) are essential for restoring the blood and immune systems after irradiation.
    • Understanding cell population dynamics aids in optimizing stem cell transplantation strategies.

    Purpose of the Study:

    • To investigate the functional differences between distinct cell populations isolated from rhesus monkey bone marrow.
    • To determine the role of specific cell fractions in lymphohemopoietic reconstitution following irradiation and transplantation.
    • To assess the presence and impact of HSC in lymphocyte-rich fractions.

    Main Methods:

    • Separation of rhesus monkey bone marrow into two heterogeneous cell populations (CP 1-7 and CP 8-10) using counterflow centrifugation-elutriation (CCE).

    Related Experiment Videos

  • Characterization of cell populations based on morphology, cell surface antigen expression, and hemopoietic progenitor cell activity.
  • Autologous transplantation studies in lethally irradiated rhesus monkeys using unfractionated bone marrow, CP 1-7, and CP 8-10.

    • Main Results:

    • CP 8-10 was concentrated with hemopoietic progenitor cell activity and HSC, effectively reconstituting the lymphohemopoietic system post-transplantation.
    • CP 1-7, rich in lymphocytes and depleted of progenitor cells, also led to eventual lymphohemopoietic reconstitution, albeit with a significant delay (up to 14 days) in engraftment.
    • The study detected HSC presence within the lymphocyte-rich, progenitor-cell-depleted fraction (CP 1-7) in the rhesus model.

    Conclusions:

    • Bone marrow fractionation via CCE yields distinct cell populations with differential roles in lymphohemopoietic reconstitution.
    • While stem cell-enriched fractions (CP 8-10) provide rapid engraftment, lymphocyte-rich fractions (CP 1-7) contain detectable HSC capable of delayed reconstitution.
    • This research highlights the complexity of bone marrow composition and the potential for HSC presence in unexpected cell fractions, informing future transplantation approaches.