Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Preferential glutamine uptake in rat brain synaptic mitochondria.

A Steib, A Rendon, J Mark

    FEBS Letters
    |October 20, 1986
    PubMed
    Summary

    Rat brain mitochondria show different glutamine uptake rates. Synaptic mitochondria exhibit higher affinity and capacity for glutamine, suggesting a key role in neurotransmitter glutamate synthesis regulation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Drug management of TB in the intensive care setting: an international multicentre study.

    The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2025
    Same author

    Observation of the ϒ(3S) Meson and Suppression of ϒ States in Pb-Pb Collisions at sqrt[s_{NN}]=5.02  TeV.

    Physical review letters·2024
    Same author

    Achievement of Target Gain Larger than Unity in an Inertial Fusion Experiment.

    Physical review letters·2024
    Same author

    Probing Small Bjorken-x Nuclear Gluonic Structure via Coherent J/ψ Photoproduction in Ultraperipheral Pb-Pb Collisions at sqrt[s_{NN}]=5.02  TeV.

    Physical review letters·2024
    Same author

    Measurement of the top quark mass using a profile likelihood approach with the lepton + jets final states in proton-proton collisions at <math></math>.

    The European physical journal. C, Particles and fields·2023
    Same author

    Observation of τ Lepton Pair Production in Ultraperipheral Pb-Pb Collisions at sqrt[s_{NN}]=5.02  TeV.

    Physical review letters·2023

    Area of Science:

    • Neuroscience
    • Mitochondrial Biology
    • Neurochemistry

    Background:

    • Mitochondria play crucial roles in cellular energy metabolism and neurotransmitter synthesis.
    • Glutamine is a key precursor for the synthesis of the excitatory neurotransmitter glutamate.
    • Differential compartmentalization of metabolic processes in the brain is essential for neuronal function.

    Purpose of the Study:

    • To investigate and compare the kinetics of glutamine uptake in isolated rat brain mitochondria from synaptic and non-synaptic origins.
    • To elucidate the transport mechanism and identify potential regulatory factors influencing glutamine uptake in different mitochondrial populations.

    Main Methods:

    • Isolation of purified rat brain mitochondria from synaptic and non-synaptic fractions.
    • Characterization of glutamine uptake using kinetic analysis (Km, Vmax).
    • Assessment of the inhibitory effects of L-glutamate and thiol-reactive agents on glutamine transport.

    Main Results:

    • Glutamine uptake in rat brain mitochondria occurs via an active, saturable transport mechanism.
    • Synaptic mitochondria display a significantly higher affinity (lower Km) and maximal transport rate (Vmax) for glutamine compared to non-synaptic mitochondria.
    • Glutamine transport is inhibited by L-glutamate and thiol-reactive reagents like mersalyl and N-ethylmaleimide.

    Conclusions:

    • Differential glutamine uptake kinetics exist between synaptic and non-synaptic brain mitochondria.
    • These differences suggest a specialized role for synaptic mitochondria in regulating neurotransmitter glutamate availability.
    • Mitochondrial glutamine transport is a potential target for modulating glutamatergic neurotransmission.

    Related Experiment Videos