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Recurrent Herpetic Stromal Keratitis in Mice, a Model for Studying Human HSK
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MYCOTIC KERATITIS.

S Gokhale1, S Joshi2, Kts Haider2

  • 1Reader, Dept of Ophthalmology, Armed Forces Medical College, Pune, 411040.

Medical Journal, Armed Forces India
|August 4, 2017
PubMed
Summary
This summary is machine-generated.

Diagnosing mycotic keratitis, a serious eye infection, is best achieved through fungal culture. Direct examination methods are less sensitive for detecting fungal elements in corneal scrapings and swabs.

Keywords:
Eye infection fungalKeratitisLaboratory diagnosis

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Area of Science:

  • Ophthalmology
  • Medical Mycology
  • Infectious Diseases

Background:

  • Mycotic keratitis is a severe fungal eye infection that can cause blindness.
  • Early and accurate diagnosis is crucial for effective treatment and preventing vision loss.

Purpose of the Study:

  • To evaluate the diagnostic sensitivity of direct microscopy versus fungal culture for suspected mycotic keratitis.
  • To identify common fungal pathogens causing keratitis in the studied population.

Main Methods:

  • A retrospective study of 52 suspected mycotic keratitis cases from January 1991 to September 1994.
  • Specimens collected via corneal scraping (32 cases) and swabs (20 cases).
  • Direct wet mount examination and fungal culture were performed on collected specimens.

Main Results:

  • Fungal elements were detected in 15.4% of direct examinations and fungi were isolated in 23.1% of cultures.
  • Corneal scraping yielded more positive results in both direct examination (5/32) and culture (7/32) compared to swabs.
  • Commonly isolated pathogens included Fusarium spp., Aspergillus spp., Candida albicans, and Cephalosporium spp.

Conclusions:

  • Fungal culture is more sensitive than direct microscopy for diagnosing mycotic keratitis.
  • Corneal scraping may be a more effective sample collection method than swabs for this diagnosis.
  • Prompt identification of fungal pathogens is essential for appropriate antifungal therapy.