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Related Concept Videos

Kidney Transplant I: Introduction01:28

Kidney Transplant I: Introduction

566
A kidney transplant is a surgical approach that involves replacing a non-functioning kidney with a healthy one from a donor. This procedure is often a treatment option for end-stage renal disease (ESRD) patients. The method requires careful recipient selection, including evaluating various medical and psychosocial factors. These criteria vary between transplant centers but generally include assessments of the patient's overall health, adherence to medical recommendations, and lifestyle...
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Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Kidney Transplant II: Surgical Procedure01:26

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Preoperative ManagementThe primary goals of preoperative management in kidney transplantation are to optimize the patient’s metabolic state and prepare them for surgery through diet adjustments, necessary dialysis, and tailored medical treatment. This phase also involves comprehensive infection screening and patient education about the surgical procedure and postoperative care to improve outcomes and adherence.Medical ManagementA comprehensive evaluation is required for both the living...
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Related Experiment Video

Updated: Feb 25, 2026

Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
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CLINICAL APPLICATION OF FLOW CYTOMETRY IN LIVE RELATED DONOR RENAL TRANSPLANTATION.

G S Chopra1, A S Narula2, S C Dash3

  • 1Classified Specialist Pathology, Army Hospital, Delhi Cantt.

Medical Journal, Armed Forces India
|August 4, 2017
PubMed
Summary
This summary is machine-generated.

Flowcytometric cross-match is a useful tool for monitoring humoral activity in renal transplant patients. This method offers objective insights into graft dysfunction, particularly in distinguishing acute rejection episodes.

Keywords:
Cross-matchFlowcytometryGraft dysfunctionKidney transplantation

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Area of Science:

  • Nephrology
  • Immunology
  • Transplantation

Background:

  • Graft dysfunction after renal transplantation can involve humoral immune activity.
  • Accurate monitoring of antidonor antibodies is crucial for successful transplantation outcomes.

Purpose of the Study:

  • To evaluate the utility of flowcytometric cross-match (FCXM) in monitoring humoral activity in renal graft dysfunction.
  • To compare FCXM with conventional cytotoxicity cross-match (CXM) assays.

Main Methods:

  • Detection of antidonor antibodies in pre- and post-transplant renal transplant recipients.
  • Comparison of FCXM and CXM assays on 52 pretransplant and post-transplant patient samples.
  • Analysis of test performance in various graft dysfunction scenarios including rejection, nephrotoxicity, and tubular necrosis.

Main Results:

  • FCXM and CXM showed similar results in most pretransplant samples, with minor discrepancies in secondary transplant cases.
  • FCXM demonstrated superior objectivity and utility over CXM in identifying 6 out of 7 mild acute graft rejection episodes post-transplant.
  • Both assays were negative in cases of cyclosporin-A nephrotoxicity, acute tubular necrosis, and most chronic rejections.

Conclusions:

  • Flowcytometric cross-match is a valuable and objective method for assessing humoral immune activity in renal transplantation.
  • FCXM enhances the ability to detect early signs of graft dysfunction, particularly mild acute rejection.
  • FCXM complements traditional methods, offering improved diagnostic capabilities in post-transplant monitoring.