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Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...

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Related Experiment Video

Updated: Feb 25, 2026

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Published on: September 7, 2018

ENZYME LINKED IMMUNOSORBENT ASSAYS REVISITED.

Mkk Rao¹, K Kapila¹, R M Gupta¹

¹Readers, Department of Microbiology, Armed Forces Medical College, Pune - 411040.

Medical Journal, Armed Forces India

|August 4, 2017

Summary

Enzyme-linked immunosorbent assay (ELISA) is a widely used immunodiagnostic tool for infectious diseases. This review covers ELISA

Area of Science:

Immunology
Infectious Diseases
Diagnostic Assays

Background:

Enzyme-linked immunosorbent assay (ELISA) is a cornerstone in the immunodiagnosis of various infectious diseases.
Continuous advancements are enhancing the sensitivity and efficacy of ELISA formulations.

Purpose of the Study:

To discuss the fundamental principles of Enzyme-linked immunosorbent assay (ELISA).
To review the application of ELISA in diagnosing tuberculosis and human immunodeficiency virus (HIV) infection.

Main Methods:

Discussion of the basic tenets and principles underlying ELISA technology.
Review of existing literature and case studies on ELISA's role in tuberculosis and HIV diagnostics.

Main Results:

Keywords:

EIA ELISA Human immunodeficiency virus Tuberculosis

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ELISA has demonstrated significant utility and wide acclaim in the immunodiagnosis of infectious diseases.

The review highlights the established role and ongoing evolution of ELISA in clinical settings.

Conclusions:

Enzyme-linked immunosorbent assay (ELISA) remains a critical and evolving tool for infectious disease diagnosis.
Its application in diagnosing tuberculosis and human immunodeficiency virus (HIV) infection is well-established and significant.