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Author Spotlight: Investigating Immune Cell Dynamics in the Tumor Microenvironment — Challenges and Innovations in Cancer Prognosis
Published on: April 12, 2024
Clinical significance of miRNA-autophagy transcript expression in patients with hepatocellular carcinoma
Marwa Matboli1, Ayman E Shafei2, Hanan H Shehata1
1Oncology Diagnostic Unit, Medical Biochemistry & Molecular biology Department, Faculty of Medicine, Ain Shams University, PO box 11381, Abbassia, Cairo, Egypt.
Aim:
This study integrates autophagy transcripts miRNAs expression based on bioinformatic analysis followed by clinical validation.
Methodology:
Cellular jun proto-oncogene mRNA, LAMP2 mRNA, miR-16 and miR-146a level were investigated in the serum and tissue of patients with hepatocellular carcinoma (HCC), chronic hepatitis C and healthy volunteers by quantitative real-time PCR. The prognostic power of this serum RNA panel was explored.
Results:
The expression of serum cellular jun proto-oncogene mRNA, LAMP2 mRNA, miR-16 and miR-146a were positive in 85.1, 94, 97.1 and 84.2% HCC patients, respectively and they were correlated with tissue levels. Our results suggested that the chosen panel is an independent prognostic factor for survival in patients with HCC.
Conclusion:
The current work provides four RNA-based biomarker panel for HCC diagnosis and prognosis.
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