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Vocal Outcomes After Cell-Based Outer Vocal Fold Implant in Porcine Bilateral Vocal Fold Resection.

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A Novel Imaging Technique to Identify Vocal Fold Scar Formation.

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Related Experiment Video

Updated: Feb 25, 2026

Construction and Characterization of a Novel Vocal Fold Bioreactor
11:11

Construction and Characterization of a Novel Vocal Fold Bioreactor

Published on: August 1, 2014

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Repairing the vibratory vocal fold.

Jennifer L Long1,2

  • 1Research Service, Greater Los Angeles VAHS, Los Angeles, California, U.S.A.

The Laryngoscope
|August 4, 2017
PubMed
Summary

A novel stem cell-based vocal fold implant (COVR) shows promise for treating vocal fold scarring and lamina propria loss. This tissue-engineered construct improved histology and vibration in rabbits, suggesting feasibility for vocal fold repair.

Keywords:
Adipose-derived stem cellmucosaphonationtissue engineeringvocal fold scarring

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Author Spotlight: Advancements in the Fabrication of Synthetic Vocal Fold Models for Phonetic and Robotic Applications
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Author Spotlight: Advancements in the Fabrication of Synthetic Vocal Fold Models for Phonetic and Robotic Applications

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Area of Science:

  • Regenerative Medicine
  • Biomaterials Science
  • Otolaryngology

Background:

  • Structural vocal fold diseases like scarring and lamina propria loss significantly impair voice quality.
  • Current treatments often have limitations in fully restoring vocal fold structure and function.
  • A vibratory vocal fold replacement could offer a new therapeutic approach.

Purpose of the Study:

  • To evaluate the feasibility and efficacy of a tissue-engineered vocal fold replacement in a rabbit model.
  • To compare the histological and functional outcomes of a cell-based implant against control groups.
  • To test the hypothesis that the implant would engraft, control wound response, reduce fibrosis, and restore vibration.

Main Methods:

  • Rabbit adipose-derived mesenchymal stem cells (ASC) were embedded in a fibrin gel to create the cell-based outer vocal fold replacement (COVR).
  • Rabbits underwent vocal fold resection and reconstruction with fibrin glue, autologous tissue, or COVR implantation.
  • Histological and phonation assessments were performed after 4 weeks.

Main Results:

  • COVR implants demonstrated successful tissue incorporation and improved histology and vibration compared to controls.
  • Graft types enhanced outcomes, with COVR implants showing better short-term vibratory results.
  • Immune reactions were observed when implanting male cells into female rabbits, suggesting sex-specific considerations.

Conclusions:

  • Stem cell-based tissue-engineered vocal fold cover replacement is feasible and beneficial in acute rabbit models.
  • The COVR implant's wound-modifying properties appear crucial in preventing fibrosis.
  • This approach holds potential for treating structural vocal fold diseases.