Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sol-gel derived B<sub>2</sub>O<sub>3</sub>-CaO borate bioactive glasses with hemostatic, antibacterial and pro-angiogenic activities.

Regenerative biomaterials·2024
Same author

Editorial: Bioengineering of biomimetic microenvironments for cardiac tissue engineering.

Frontiers in bioengineering and biotechnology·2023
Same author

The Impact of 45S5-Bioactive Glass on Synovial Cells in Knee Osteoarthritis-An In Vitro Study.

Materials (Basel, Switzerland)·2023
Same author

Bioactive Glass and Silica Particles for Skeletal and Cardiac Muscle Tissue Regeneration.

Tissue engineering. Part B, Reviews·2023
Same author

Surface engineering of mesoporous bioactive glass nanoparticles with bacteriophages for enhanced antibacterial activity.

Colloids and surfaces. B, Biointerfaces·2023
Same author

Mending a broken heart by biomimetic 3D printed natural biomaterial-based cardiac patches: a review.

Frontiers in bioengineering and biotechnology·2023

Related Experiment Video

Updated: Feb 25, 2026

Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer
07:05

Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer

Published on: September 22, 2015

10.5K

Fabrication of Cell-Loaded Two-Phase 3D Constructs for Tissue Engineering.

Tobias Zehnder1, Tim Freund2, Merve Demir3

  • 1Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Cauerstraße 6, Erlangen 91058, Germany. tobias.zehnder@fau.de.

Materials (Basel, Switzerland)
|August 5, 2017
PubMed
Summary

This study combined thermoplastic and hydrogel materials for 3D tissue engineering scaffolds. The hybrid materials showed promising cell proliferation and degradation, indicating potential for advanced biofabrication.

Keywords:
alginate dialdehydebiofabricationgelatinehydrogelspolycaprolactonesequential bioplottingtissue engineering

More Related Videos

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
09:24

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

15.2K
Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures
05:52

Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures

Published on: September 27, 2019

10.0K

Related Experiment Videos

Last Updated: Feb 25, 2026

Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer
07:05

Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer

Published on: September 22, 2015

10.5K
Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
09:24

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

15.2K
Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures
05:52

Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures

Published on: September 27, 2019

10.0K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Additive Manufacturing

Background:

  • Optimizing hydrogels for biofabrication requires balancing shape stability, mechanical properties, and cell response.
  • Combining additive manufacturing techniques, like hot-melt extrusion and bioplotting, offers a novel approach to create complex 3D constructs.

Purpose of the Study:

  • To investigate the potential of a hybrid polycaprolactone (PCL) and polyethylene glycol (PEG) blend with alginate dialdehyde gelatine (ADA-GEL) hydrogel for 3D tissue engineering scaffolds.
  • To evaluate the plotting properties, scaffold characteristics, and cell response of the PCL-PEG blend and the hybrid constructs.

Main Methods:

  • Fabrication of 3D scaffolds using sequential plotting of PCL-PEG blends and cell-laden ADA-GEL hydrogels.
  • Characterization of PCL-PEG blends for miscibility, wetting, and cell response.
  • Evaluation of scaffold properties including pore size, porosity, strut width, degradation, and mechanical stability.
  • Analysis of cell viability, distribution, morphology, and interaction within the hybrid constructs.

Main Results:

  • PCL-PEG blends demonstrated improved hydrophilicity and cell response with increasing PEG content.
  • PEG blending enhanced scaffold degradation while reducing mechanical properties.
  • Hybrid constructs supported cell viability, distribution, and proliferation within both hydrogel and thermoplastic phases.
  • The thermoplastic support structure exhibited increased degradation, facilitating cell interaction and proliferation.

Conclusions:

  • The novel combination of PCL-PEG blends and ADA-GEL hydrogels shows significant potential for biofabricating 3D tissue engineering scaffolds.
  • The material system supports cell proliferation and interaction, with tunable degradation characteristics of the thermoplastic support.
  • This hybrid approach offers a promising strategy for developing advanced, functional tissue engineering constructs.