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Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging
Published on: August 1, 2017
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Scaffold design for artificial tissue with bone marrow stem cells
Aurelija Noreikaitė1, Ieva Antanavičiūtė2, Valeryia Mikalayeva2
1Institute of Physiology and Pharmacology, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania.
Medicina (Kaunas, Lithuania)
|August 5, 2017
Summary
Polymeric materials like polylactic acid (PLA) and fibrin support cell growth in scaffolds, while polyimide film (PI) can act as a barrier. Electrospinning and laser structuring enable advanced multi-layer scaffold development for tissue engineering.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Cell Biology
Background:
- Developing artificial scaffolds is crucial for tissue regeneration and understanding cell behavior.
- Polymeric materials offer diverse properties for scaffold fabrication, influencing cell integration and function.
Purpose of the Study:
- To evaluate collagen, fibrin, polyimide film (PI), and polylactic acid (PLA) for single- and multi-layer scaffold formation.
- To assess cell incorporation, proliferation, and migration within various scaffold materials.
Main Methods:
- Utilized rabbit bone marrow stem cells (rBMSCs) and human mesenchymal stem cells (hMSCs).
- Fabricated scaffolds from collagen, fibrin, electrospun PLA, and PI film, including PI/PLA combinations.
- Employed cell imaging, histological analysis, and time-lapse microscopy to evaluate cell behavior.
Main Results:
- Fibrin scaffolds showed complete cell penetration, unlike collagen scaffolds with limited external layer colonization.
- Human mesenchymal stem cells (hMSCs) exhibited comparable mobility on smooth and micro-structured PI films (18-20 μm/h).
- Electrospun PLA scaffolds (100 μm thick) allowed complete cell spreading within 3 days; PI/PLA multilayer scaffolds supported cell growth.
Conclusions:
- Electrospinning and laser micro-structuring are promising for developing advanced multi-layer scaffolds.
- PLA, fibrin, and collagen are suitable for cell inhabitation and as inner scaffold layers.
- PI shows potential as a barrier layer to control cell migration; further optimization is required.
