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Related Experiment Videos

Sequence analysis of the cDNA encoding human liver glycogen phosphorylase reveals tissue-specific codon usage.

C B Newgard, K Nakano, P K Hwang

    Proceedings of the National Academy of Sciences of the United States of America
    |November 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

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    Researchers cloned human liver glycogen phosphorylase cDNA, revealing significant differences in G+C content and codon usage compared to rabbit muscle glycogen phosphorylase. This highlights tissue-specific biases in gene sequences.

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Genetics

    Background:

    • Glycogen phosphorylase is a key enzyme in glycogen metabolism.
    • Tissue-specific isoforms of glycogen phosphorylase exist, but their molecular basis is not fully understood.

    Purpose of the Study:

    • To clone and sequence human liver glycogen phosphorylase cDNA.
    • To compare the human liver sequence with known muscle glycogen phosphorylase sequences.
    • To investigate potential differences in codon usage and G+C content between liver and muscle isoforms.

    Main Methods:

    • cDNA cloning of human liver glycogen phosphorylase.
    • Northern blot analysis to examine mRNA expression in different tissues.
    • Nucleotide sequencing of the cloned cDNA.

    Related Experiment Videos

  • Bioinformatic analysis and comparison with rabbit muscle phosphorylase sequence.

    • Main Results:

    • Successfully cloned and sequenced human liver glycogen phosphorylase cDNA.
    • Blot-hybridization confirmed preferential expression in liver tissue.
    • Despite 80% amino acid identity, significant divergence in G+C content and codon usage was observed between human liver and rabbit muscle phosphorylase cDNAs.
    • Human liver phosphorylase cDNA showed a mosaic pattern, with a highly G+C-rich N-terminal region.

    Conclusions:

    • Human liver and muscle glycogen phosphorylase cDNAs exhibit distinct G+C content and codon usage patterns.
    • These findings suggest a general bias in codon usage between liver and muscle tissues in mammals.
    • The observed sequence divergence may have implications for gene expression regulation and protein evolution.