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METHODS FOR ESTIMATION OF BLOOD GLUCOSE : A COMPARATIVE EVALUATION.

Vivek N Ambade1, Y V Sharma2, B L Somani3

  • 1Scientist C, Armed Forces Medical College, Pune 411040.

Medical Journal, Armed Forces India
|August 5, 2017
PubMed
Summary

The GOD-POD method for blood glucose testing is accurate and precise, outperforming older methods like Modified Folin Wu and O-Toluidine. This makes it ideal for routine clinical chemistry analysis.

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Area of Science:

  • Clinical Chemistry
  • Biomedical Analysis

Background:

  • Blood glucose measurement is a critical clinical chemistry parameter.
  • Traditional methods like Modified Folin Wu and O-Toluidine are commonly used but have limitations.

Purpose of the Study:

  • To compare the accuracy and precision of Modified Folin Wu, O-Toluidine, and GOD-POD methods against the reference UV-Hexokinase method for blood glucose analysis.

Main Methods:

  • Comparative study involving Modified Folin Wu, O-Toluidine, GOD-POD, and UV-Hexokinase methods.
  • Evaluation of linearity, precision (Coefficient of Variation - CV), and accuracy (Average Deviation).

Main Results:

  • Modified Folin Wu and O-Toluidine methods exhibited upward deviation, imprecision (CVs 4.9%-3.5% and 6%-5.8%), and inaccuracy (Avg. deviation 5.76% and 10.68%).
Keywords:
Blood glucoseDiabetes mellitusGlucose oxidase

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  • The GOD-POD method demonstrated linearity up to 500 mg/dl, good precision (CV 0.7%-1.4%), and high accuracy (Avg. deviation -0.97%).

    • Conclusions:

    • The GOD-POD method is a superior alternative for routine blood glucose analysis due to its accuracy, precision, simplicity, and cost-effectiveness.
    • The GOD-POD method is recommended for adoption in routine clinical chemistry laboratories.