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Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
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Updated: Feb 25, 2026

TransFLP &#x2014; A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination
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PLASMID MEDIATED DRUG RESISTANCE IN VIBRIO CHOLERAE 0139 BENGAL.

R N Misra1, N K Debata2, A Nagend1

  • 1Reader, Department of Microbiology, Armed Forces Medical College, Pune-40.

Medical Journal, Armed Forces India
|August 5, 2017
PubMed
Summary

Vibrio cholerae O139 Bengal strains show resistance to common antibiotics like ampicillin and trimethoprim. This antibiotic resistance in cholera bacteria can be transferred to other bacteria, highlighting a public health concern.

Keywords:
CAMP testDrug resistancePlasmidVibrio cholerae 0139.

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Area of Science:

  • Microbiology
  • Infectious Diseases
  • Molecular Biology

Background:

  • Diarrhea outbreaks are often caused by Vibrio cholerae.
  • Understanding the antibiotic resistance patterns of different Vibrio cholerae strains is crucial for effective treatment.

Purpose of the Study:

  • To identify Vibrio cholerae strains from diarrhea cases.
  • To determine the antibiotic susceptibility of Vibrio cholerae O139 Bengal and El Tor strains.
  • To investigate the transferability of antibiotic resistance.

Main Methods:

  • Isolation and identification of Vibrio cholerae strains using biochemical and serological tests.
  • Modified CAMP reaction for hemolysis assessment.
  • Agar dilution method to determine minimum inhibitory concentrations (MICs).
  • Conjugation experiments with E. coli K-12.
  • Agarose gel electrophoresis for plasmid analysis.

Main Results:

  • 12 (75%) strains were identified as Vibrio cholerae O139 Bengal, and 4 (25%) as Vibrio cholerae El Tor.
  • Vibrio cholerae O139 strains were resistant to ampicillin, streptomycin, chloramphenicol, sulphamethoxazole, and trimethoprim.
  • All strains were sensitive to norfloxacin and cefotaxime.
  • Antibiotic resistance genes were transferable via conjugation, with distinct plasmid bands observed.

Conclusions:

  • Vibrio cholerae O139 Bengal strains exhibit significant resistance to multiple antibiotics.
  • The transferability of resistance suggests a potential for wider dissemination of antibiotic resistance.
  • Novel antibiotics like norfloxacin and cefotaxime remain effective against these strains.