Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

4.2K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.2K
RNA-seq03:21

RNA-seq

12.2K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.2K
Magnetic Resonance Imaging01:24

Magnetic Resonance Imaging

10.0K
Magnetic resonance imaging (MRI) is a noninvasive medical imaging technique based on a phenomenon of nuclear physics discovered in the 1930s, in which matter exposed to magnetic fields and radio waves was found to emit radio signals. In 1970, a physician and researcher named Raymond Damadian noticed that malignant (cancerous) tissue gave off different signals than normal body tissue. He applied for a patent for the first MRI scanning device in clinical use by the early 1980s. The early MRI...
10.0K
Proteomics01:33

Proteomics

10.0K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
10.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Histidine Ethylation by Histidine Methyltransferases SETD3 and METTL9.

Chembiochem : a European journal of chemical biology·2026
Same author

Small-Molecule Activation of mRNA Translation by Click-to-Release Reaction in Cells.

Angewandte Chemie (International ed. in English)·2026
Same author

The 5' Cap Epitranscriptome and Beyond: Natural and Engineered 5' Cap Modifications for Optimizing mRNA Therapeutics and Functional Studies.

ChemMedChem·2026
Same author

Histidine methylation <i>via</i> an enzymatic cascade with <i>in situ</i> generation of nucleoside-modified AdoMet analogues.

Chemical communications (Cambridge, England)·2026
Same author

Structure-guided engineering of a polyphosphate kinase 2 class III from an <i>Erysipelotrichaceae</i> bacterium to produce base-modified purine nucleotides.

RSC chemical biology·2025
Same author

Light-controlled genome editing by activation of Cas9-mRNA translation.

Chemical science·2025

Related Experiment Video

Updated: Feb 25, 2026

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
12:20

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

12.2K

Making the Message Clear: Concepts for mRNA Imaging.

Kristina Rau1, Andrea Rentmeister1

  • 1Institute of Biochemistry, University of Münster, Wilhelm-Klemm-Strasse 2, 48149 Münster, Germany.

ACS Central Science
|August 5, 2017
PubMed
Summary

Understanding RNA dynamics requires advanced imaging. This perspective reviews cutting-edge RNA labeling and imaging techniques for visualizing RNA distribution and trafficking within living cells.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biophysics

Background:

  • Standard transcriptome analysis quantifies gene expression levels.
  • Cellular RNA molecules are subject to complex regulatory mechanisms.
  • Understanding RNA spatiotemporal distribution is crucial for cell function.

Purpose of the Study:

  • To review and compare state-of-the-art RNA imaging methods.
  • To highlight the strengths and weaknesses of various RNA visualization techniques.
  • To emphasize the importance of live-cell RNA imaging for studying RNA biology.

Main Methods:

  • RNA labeling techniques (e.g., fluorescent probes, genetic tags).
  • High-resolution microscopy (e.g., confocal, super-resolution).
  • Live-cell imaging protocols.

More Related Videos

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

445
Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers
07:39

Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers

Published on: June 4, 2019

8.0K

Related Experiment Videos

Last Updated: Feb 25, 2026

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
12:20

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

12.2K
MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

445
Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers
07:39

Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers

Published on: June 4, 2019

8.0K

Main Results:

  • Overview of current RNA imaging technologies.
  • Discussion of method-specific advantages and limitations.
  • Identification of key challenges and future directions in the field.

Conclusions:

  • RNA imaging provides critical insights beyond standard expression analysis.
  • Live-cell RNA visualization is essential for understanding RNA dynamics.
  • Advancements in RNA imaging will accelerate discoveries in molecular and cell biology.