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Chemical Shift: Internal References and Solvent Effects

Chemical Shift: Internal References and Solvent Effects

In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...

NMR Spectrometers: Overview

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NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...

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Related Experiment Video

Updated: Feb 25, 2026

A Tuberculosis Molecular Bacterial Load Assay TB-MBLA

A Tuberculosis Molecular Bacterial Load Assay TB-MBLA

Published on: April 30, 2020

Who Needs Reference Materials?

Melissa Phillips¹, Håkan Emteborg²

¹National Institute of Standards and Technology, Gaithersburg, MD, melissa.phillips@nist.gov.

Journal of AOAC International

|August 5, 2017

Summary

No abstract available in PubMed .

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