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Related Experiment Video

Updated: Feb 25, 2026

Preparation of DNA-crosslinked Polyacrylamide Hydrogels

Preparation of DNA-crosslinked Polyacrylamide Hydrogels

Published on: August 27, 2014

Intelligent, Biodegradable, and Self-Healing Hydrogels Utilizing DNA Quadruplexes.

Shizuma Tanaka¹, Kenta Wakabayashi¹, Kazuki Fukushima¹

¹Department of Chemistry and Materials Engineering, Kansai University, 3-3-35 Yamate, Suita, Osaka, 564-8680, Japan.

Chemistry, an Asian Journal

|August 5, 2017

Summary

Researchers developed intelligent, biodegradable DNA quadruplex hydrogels. These responsive gels form rapidly and offer potential as injectable polymers with self-healing properties.

Keywords:

DNA gels injectable polymers polyethylene glycol quadruplexes

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Last Updated: Feb 25, 2026

Preparation of DNA-crosslinked Polyacrylamide Hydrogels

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Published on: August 27, 2014

Synthesis of an Intein-mediated Artificial Protein Hydrogel

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Published on: January 27, 2014

Fabricating Degradable Thermoresponsive Hydrogels on Multiple Length Scales via Reactive Extrusion, Microfluidics, Self-assembly, and Electrospinning

Fabricating Degradable Thermoresponsive Hydrogels on Multiple Length Scales via Reactive Extrusion, Microfluidics, Self-assembly, and Electrospinning

Published on: April 16, 2018

Area of Science:

Biomaterials Science
Polymer Chemistry
Supramolecular Chemistry

Background:

Hydrogels are versatile materials with applications in drug delivery and tissue engineering.
Stimuli-responsive hydrogels offer dynamic control over material properties.
DNA-based materials are gaining attention for their programmability and biocompatibility.

Purpose of the Study:

To synthesize a novel class of DNA-based hydrogels.
To investigate the stimuli-responsive behavior of these DNA quadruplex hydrogels.
To evaluate the potential of these hydrogels as injectable polymers.

Main Methods:

Synthesis of DNA-PEG-DNA triblock copolymers via coupling deoxynucleotide phosphoramidite monomers to polyethylene glycols (PEGs).
Induction of hydrogel formation through DNA quadruplex assembly triggered by ions (Na+, K+) or complementary DNA strands.
Characterization of gelation kinetics, thermal stability, rheological properties, and self-healing capabilities.

Main Results:

Successful construction of DNA quadruplex hydrogels using DNA-PEG-DNA triblock copolymers.
Rapid gelation (seconds) upon addition of specific triggers, similar to calcium-alginate hydrogels.
Demonstrated responsiveness to Na+, K+, and complementary DNA strands.
Hydrogels exhibited good thermal stability, favorable rheological properties, and self-healing ability.
Efficient synthesis yielding approximately 10 grams of DNA-PEG conjugates.

Conclusions:

A new class of intelligent and biodegradable DNA quadruplex hydrogels has been developed.
These hydrogels show promise as injectable polymers due to rapid gelation and tunable responsiveness.
The self-healing and stable properties suggest broad applicability in biomedical fields.