Postcolumn determination of polyphenolic antioxidants in Cirsium vulgare (Savi) Ten. extracts

Novel methods for the determination of polyphenolic antioxidants present in extracts from inflorescences of Cirsium vulgare (Savi) Ten. based on ultra-high performance liquid chromatography with photodiode array and chemiluminescence detection have been developed. Under the optimized conditions of chromatographic separation the analytical characteristic of the method was performed. The proposed method was successfully applied to the determination of ten polyphenols present in inflorescences of Cirsium vulgare. A comparison of the contents of analytes in extracts prepared by using various extraction media (methanol, ethanol, 70% methanol, 70% ethanol, and water) was carried out for the first time. For the postcolumn detection of scavenging activity of polyphenolic antioxidants against reactive oxygen species (H₂ O₂ , ^• OH, O₂^{• -} ) three systems based on chemiluminescence of luminol were used. A review of the current scientific literature shows that this is the first report on the application of luminol-based postcolumn detection for the on-line investigation of ^• OH scavenging activity. The main compound determined in extracts from inflorescences of Cirsium vulgare was apigenin 7-O-glucuronide, whereas the highest antioxidant activity was observed for chlorogenic acid, luteolin 7-O-glucoside, and apigenin.