The MEMbrane Protein Single ShoT Amplification Recipe: MemStar

Here, we present a simple overexpression condition for high-throughput screening of membrane proteins in Escherichia coli. For the vast majority of bacterial membrane protein targets tested the MEMbrane protein Single shoT Amplification Recipe-MemStar-leads to high production yields of target protein. The use of MemStar has facilitated structural studies of several transport proteins.