Janus enantiomorphous nanomaterial assembly on substrate surfaces for chirality-dependent cell adhesion

A janus enantiomorphous nanomaterial assembly on substrate surfaces is prepared by depositing two different enantiomorphous nanomaterials (NMs) onto two different halves of the same glass substrate. This advanced construct allows us to simultaneously study and compare how the opposite biopolymer enantiomers functionalized onto the NM surfaces affect cell adhesion under exactly the same reaction conditions and at the same time, using a single biomaterial. Cell experiments show that cell adhesion to the Janus enantiomorphous NM assembly is chirality dependent when opposite enantiomers are examined on a single biomaterial. These results are more pronounced in serum-containing media, indicating the positive effect of protein adsorption on cell-chiral biomolecule functionalized NM surface interactions.