Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

10.0K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
10.0K
Skeletal Muscle Anatomy00:55

Skeletal Muscle Anatomy

93.1K
Skeletal muscle is the most abundant type of muscle in the body. Tendons are the connective tissue that attaches skeletal muscle to bones. Skeletal muscles pull on tendons, which in turn pull on bones to carry out voluntary movements.
93.1K
Overview of Skeletal Muscle01:15

Overview of Skeletal Muscle

15.6K
Skeletal muscles are composed of a bundle of muscle fibers and are attached to bones through tendons. Each skeletal muscle fiber is a single muscle cell. The sarcolemma, the plasma membrane of a skeletal muscle cell, consists of a lipid bilayer and glycocalyx that supports muscle fibers. The sarcolemma extends into the muscle cells to form tubular structures called transverse or T-tubules. Each side of the T-tubules consists of a membrane-bound structure called the sarcoplasmic reticulum,...
15.6K
Classification of Skeletal Muscle Fibers01:48

Classification of Skeletal Muscle Fibers

59.7K
Skeletal muscles continuously produce ATP to provide the energy that enables muscle contractions. Skeletal muscle fibers can be categorized into three types based on differences in their contraction speed and how they produce ATP, as well as physical differences related to these factors. Most human muscles contain all three muscle fiber types, albeit in varying proportions.
Slow-Twitch Muscle Fibers
Slow oxidative, muscle fibers appear red due to large numbers of capillaries and high levels of...
59.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Proteome analysis refines molecular processes underlying metamorphosis in the ascidian Ciona intestinalis.

PloS one·2026
Same author

Proteographâ„¢-based proteome and sphingolipidome analyses identified novel serum biomarkers to monitor astronauts' health in spaceflight.

Frontiers in physiology·2026
Same author

Post-mortem forensic application of proteomics on human ribs: Investigating the phenomenon of vital reaction.

International journal of legal medicine·2025
Same author

Correction: Proteomic screening identifies PF4/Cxcl4 as a critical driver of myelofibrosis.

Leukemia·2025
Same author

Muscle Proteome Analysis of Facioscapulohumeral Dystrophy Patients Reveals a Metabolic Rewiring Promoting Oxidative/Reductive Stress Contributing to the Loss of Muscle Function.

Antioxidants (Basel, Switzerland)·2024
Same author

Mass spectrometry proteomic profiling of postmortem human muscle degradation for PMI estimation.

Forensic science international·2024

Related Experiment Video

Updated: Feb 25, 2026

Skeletal Muscle Gender Dimorphism from Proteomics
09:29

Skeletal Muscle Gender Dimorphism from Proteomics

Published on: December 14, 2011

13.0K

Mapping the human skeletal muscle proteome: progress and potential.

Daniele Capitanio1, Manuela Moriggi1, Cecilia Gelfi1

  • 1a Department of Biomedical Sciences for Health , University of Milan , Segrate , Milan , Italy.

Expert Review of Proteomics
|August 8, 2017
PubMed
Summary

Understanding human skeletal muscle proteome is crucial for health and disease. This review highlights challenges and future directions for muscle research, focusing on improved diagnostic methods and protein characterization.

Keywords:
2-D DIGELC-MS/MSSkeletal muscleexercisehypoxiaiTRAQ labellingmass spectrometrymuscular dystrophiessarcopenia

More Related Videos

Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues
09:30

Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues

Published on: February 18, 2021

4.8K
TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
07:44

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Published on: June 8, 2020

13.4K

Related Experiment Videos

Last Updated: Feb 25, 2026

Skeletal Muscle Gender Dimorphism from Proteomics
09:29

Skeletal Muscle Gender Dimorphism from Proteomics

Published on: December 14, 2011

13.0K
Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues
09:30

Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues

Published on: February 18, 2021

4.8K
TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
07:44

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Published on: June 8, 2020

13.4K

Area of Science:

  • Human skeletal muscle biology
  • Proteomics
  • Physiological and pathological states

Background:

  • Human skeletal muscle constitutes 40% of body mass, yet its proteome is complex and challenging to analyze.
  • Inter-individual variability, diverse muscle types, and wide protein dynamic ranges necessitate advanced methodologies.
  • Ethical concerns and biopsy limitations hinder physiological studies, especially for comparative analyses.

Purpose of the Study:

  • To critically analyze existing muscle proteome studies.
  • To identify knowledge gaps in physiological and pathological muscle conditions.
  • To propose future research directions for muscle function and disorders.

Main Methods:

  • Critical review of published muscle proteome research.
  • Analysis of challenges in muscle tissue and biological fluid analysis.
  • Identification of methodological limitations in current proteomic approaches.

Main Results:

  • Significant gaps remain in understanding muscle proteome in states like training, aging, metabolic disorders, and muscular dystrophies.
  • Current methodologies face challenges in characterizing intact proteins and post-translational modifications.
  • Biological fluid analysis offers potential for improved diagnosis and monitoring.

Conclusions:

  • Future efforts should focus on analyzing muscle-derived fragments in biological fluids for better diagnosis and monitoring.
  • Methodological advancements are needed for improved characterization of intact proteins and post-translational modifications.
  • Enhanced understanding of the muscle proteome is key to unraveling molecular mechanisms underlying muscle disorders.