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Related Concept Videos

Autophagy01:27

Autophagy

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Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
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Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

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Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
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Autophagic Cell Death01:18

Autophagic Cell Death

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Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
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Stem Cell Niche01:26

Stem Cell Niche

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The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
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Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

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The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
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Mitochondria01:37

Mitochondria

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Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
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Related Experiment Video

Updated: Feb 25, 2026

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells
08:35

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells

Published on: June 12, 2017

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Autophagy in stem cell aging.

Miren Revuelta1, Ander Matheu1,2

  • 1Cellular Oncology Group, Biodonostia Institute, San Sebastian, Spain.

Aging Cell
|August 8, 2017
PubMed
Summary
This summary is machine-generated.

Autophagy, a cellular cleaning process, is crucial for stem cell health during aging. Impaired autophagy leads to stem cell exhaustion and contributes to age-related diseases.

Keywords:
HSCMSCagingautophagystem cell

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Related Experiment Videos

Last Updated: Feb 25, 2026

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells
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Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo

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Area of Science:

  • Cellular Biology
  • Aging Research
  • Stem Cell Science

Background:

  • Aging causes tissue imbalance and reduced organ regeneration due to stem cell exhaustion.
  • Autophagy, a key cellular process, degrades damaged components and is vital for maintaining stem cell function.
  • Declining autophagy is a hallmark of aging, impacting stem cell number and functionality.

Purpose of the Study:

  • To review recent findings on the role of autophagy in stem cell activity and aging.
  • To elucidate the impact of impaired autophagy on stem cell exhaustion and age-associated diseases.

Main Methods:

  • Review of two independent studies focusing on muscle and hematopoietic stem cells.
  • Analysis of the relationship between autophagy impairment and stem cell aging.

Main Results:

  • Stem cells rely on autophagy to clear cellular waste during quiescence.
  • Impaired autophagy directly contributes to stem cell exhaustion in both muscle and hematopoietic stem cells.
  • Autophagy dysfunction is linked to the aging process and age-related diseases.

Conclusions:

  • Autophagy is essential for maintaining stem cell health and regenerative capacity throughout the aging process.
  • Understanding autophagy's role is critical for developing interventions against age-associated decline and diseases.