Characterization of the continuous green monkey spleen cell line 455

A continuous line of adult green monkey spleen cells has been established and designated 455 (according to the number of animal). Cell suspension for primary explantation has been prepared by perfusion and disaggregation of the organ. The obtained culture, which consisted of fibroblast-like cells with chromosome modal number 60, underwent 25 passages followed by ageing and death (line 455 D). At passage level 13 in 2 culture flasks with 455 D cells, islets of polygonal cells had developed within 35 days; they gave rise to a continuous cell line with chromosome modal number 110 (designated line 455). Line 455 has a high proliferative activity but low demands on the composition of culture media and it can be easily passaged with the bovine serum at concentration of 1-5%. It is highly sensitive to a number of viruses. It is not contaminated with bacteria, fungi, mycoplasmas or viruses, it does not have a tumourigenic activity. Hence, it is a promising cellular model for virological studies, in particular, for preparation of inactivated vaccines along with Vero and 4647 cells. The line 455 can be used for investigation of the problems of haemopoiesis and immunogenesis.