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Sequence complementarity at the ribosomal Peptidyl Transferase Centre implies self-replicating origin.

Ilana Agmon1,2

  • 1Institute for Advanced Studies in Theoretical Chemistry, Schulich Faculty of Chemistry, Technion - Israel Institute of Technology, Haifa, Israel.

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|August 9, 2017
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Summary

The study reveals nucleotide complementarity in bacterial ribosomes, suggesting a simple, self-replicating proto-ribosome model. This finding offers a potential mechanism for early life

Keywords:
RNAorigin of liferibosome evolutionself-replicationsymmetrical region

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Area of Science:

  • Origin of life studies
  • Molecular biology
  • Biochemistry

Background:

  • The emergence of life necessitates a self-replicating proto-ribosome capable of catalyzing peptide formation.
  • Existing proto-ribosome models derived from the Peptidyl Transferase Centre (PTC) face challenges due to poor prebiotic replication capabilities.

Purpose of the Study:

  • To investigate the structural basis for proto-ribosome replication.
  • To explore a potential self-replication mechanism for early RNA-based life.

Main Methods:

  • Analysis of nucleotide complementarity within the Peptidyl Transferase Centre (PTC) of bacterial ribosomes.
  • Structural examination of the PTC cavity to infer dimeric organization.

Main Results:

  • Demonstrated complementarity between nucleotides forming the two halves of the PTC cavity in bacterial ribosomes.
  • Evidence supporting a dimeric structure for the proto-ribosome, explaining the PTC's symmetry.
  • Identification of a plausible self-replication mechanism where each monomer strand serves as a template for its counterpart.

Conclusions:

  • The observed nucleotide complementarity supports a dimeric proto-ribosome model.
  • This complementarity indicates a simple and efficient self-replication strategy for early ribozymes.
  • The findings provide a feasible scenario for the emergence and sustainability of early life through self-replicating RNA structures.