Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

2.1K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
2.1K
CRISPR01:59

CRISPR

58.2K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.2K
Epigenetic Regulation01:46

Epigenetic Regulation

34.0K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
34.0K
Epigenetic Regulation01:37

Epigenetic Regulation

4.0K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
4.0K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.9K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Epicardial adipose tissue density, myocardial fibrosis, and heart failure with preserved ejection fraction in postmenopausal women: a mediation analysis.

Quantitative imaging in medicine and surgery·2026
Same author

Spontaneous formation of highly concentrated phenylacetylcarbinol via in situ phase separation in deep eutectic solvents using Candida magnoliae whole cells.

Scientific reports·2026
Same author

Daily Associations Between Perceived Maternal Privacy Invasion and Youth Information Management: How Do Cultural Factors Matter?

Journal of adolescence·2026
Same author

Physicochemical and Structural Characterization of Composite Gels of Commercial Hemp Seed Protein Concentrate and Hemp Seed Protein Hydrolysate.

Gels (Basel, Switzerland)·2026
Same author

High-throughput machine learning-aided antibody discovery for cell surface antigens.

Cell systems·2026
Same author

Targeting SRSF6 to Enhance Cisplatin Sensitivity by Modulating Redox Balance via NFE2L1 exon 4 Splicing in ESCC.

International journal of biological sciences·2026

Related Experiment Video

Updated: Feb 25, 2026

An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling
08:34

An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling

Published on: December 18, 2017

7.1K

Chemically Controlled Epigenome Editing through an Inducible dCas9 System.

Tingjun Chen1, Dan Gao1, Roushu Zhang1

  • 1Department of Chemistry and Chemical Biology, University of New Mexico , 300 Terrace Street NE, Albuquerque, New Mexico 87131, United States.

Journal of the American Chemical Society
|August 9, 2017
PubMed
Summary

We developed a new inducible system to precisely control histone acetylation at specific gene locations. This tool helps understand the direct link between histone modifications and gene activation timing and stability.

More Related Videos

Epigenetic Engineering of K562 Cells: Dual-Vector Episomal Strategy for Stable Targeted DNA Methylation using dCas9-DNMT3A and -HDAC1 Fusion Proteins
09:56

Epigenetic Engineering of K562 Cells: Dual-Vector Episomal Strategy for Stable Targeted DNA Methylation using dCas9-DNMT3A and -HDAC1 Fusion Proteins

Published on: October 31, 2025

512
Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

7.0K

Related Experiment Videos

Last Updated: Feb 25, 2026

An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling
08:34

An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling

Published on: December 18, 2017

7.1K
Epigenetic Engineering of K562 Cells: Dual-Vector Episomal Strategy for Stable Targeted DNA Methylation using dCas9-DNMT3A and -HDAC1 Fusion Proteins
09:56

Epigenetic Engineering of K562 Cells: Dual-Vector Episomal Strategy for Stable Targeted DNA Methylation using dCas9-DNMT3A and -HDAC1 Fusion Proteins

Published on: October 31, 2025

512
Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

7.0K

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Gene Regulation

Background:

  • Histone modifications play a crucial role in regulating gene activity.
  • Establishing direct causal links between specific histone modifications and gene expression has been challenging due to technical limitations.

Purpose of the Study:

  • To develop an inducible system for precise control and study of histone modifications.
  • To investigate the temporal relationship between histone acetylation and gene activation.
  • To assess the stability of induced histone modifications.

Main Methods:

  • Development of an inducible system integrating dCas9-based targeting and chemically induced proximity.
  • Recruitment of P300 acetyltransferase to specific gene loci using small molecules.
  • Acetylation of histone H3 at lysine 27 (H3K27) at targeted genomic sites.
  • Analysis of temporal dynamics of histone acetylation and gene activation.

Main Results:

  • Successfully established a system for targeted and inducible histone acetylation at specific gene loci.
  • Elucidated the precise temporal order of H3K27 acetylation and subsequent gene activation.
  • Demonstrated the stability of the induced histone modification in cells.

Conclusions:

  • The developed inducible system provides a powerful tool for dissecting causal relationships in epigenetics.
  • Understanding the temporal dynamics of histone modifications is key to comprehending gene regulation.
  • This technology facilitates the study of epigenetic mechanisms with high precision.