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Related Concept Videos

Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

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Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
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Labeling DNA Probes03:31

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Related Experiment Video

Updated: Feb 25, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
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Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

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Simple method for self-referenced and lable-free biosensing by using a capillary sensing element.

Yun Liu, Shimeng Chen, Qiang Liu

    Optics Express
    |August 10, 2017
    PubMed
    Summary
    This summary is machine-generated.

    We developed a simple, label-free biosensing method using a capillary and webcam to detect biological information. This approach offers accurate, reference-compensated measurements for various applications.

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    Area of Science:

    • Optoelectronics
    • Biosensing
    • Surface Plasmon Resonance (SPR) imaging

    Background:

    • Label-free biosensing is crucial for real-time biological analysis.
    • Existing Surface Plasmon Resonance (SPR) methods often require complex instrumentation and calibration.
    • Developing simple, cost-effective, and reference-compensated biosensing platforms is essential.

    Purpose of the Study:

    • To demonstrate a simple, self-reference, and label-free biosensing method.
    • To utilize a capillary sensing element with common optoelectronic devices (LED and webcam).
    • To achieve accurate detection with effective compensation for environmental and instrumental fluctuations.

    Main Methods:

    • A capillary sensing element with a functionalized gold film was illuminated by an LED and detected by a webcam.
    • Regions of Interest (ROIs) were defined for measurement and reference channels for image processing.
    • Surface Plasmon Resonance (SPR) modes were excited and analyzed for biological information extraction.

    Main Results:

    • The biosensing device achieved a sensitivity of 1145%/RIU and a resolution better than 5.287 × 10-4 RIU.
    • Effective compensation for temperature, bulk refractive index (RI), and light source fluctuations was demonstrated.
    • The method showed an approximately linear response for concanavalin A (Con A) concentration measurements.

    Conclusions:

    • The developed method offers a simple and effective approach for multichannel SPR sensing.
    • It provides a new strategy for reference-compensated calibration in label-free SPR detection.
    • This technique presents a promising alternative for sensitive and accurate biological measurements.