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Related Experiment Video

Updated: Feb 25, 2026

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
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Speckle reduction in quantitative phase imaging by generating spatially incoherent laser field at electroactive

Hamid Farrokhi, Jeeranan Boonruangkan, Byung Jae Chun

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    We developed a speckle reduction method for quantitative phase imaging (QPI) using optical diffusers. This technique enhances lateral resolution and reduces artifacts in 3D phase imaging of transparent objects.

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    Area of Science:

    • Optical imaging
    • Biomedical optics
    • Phase contrast microscopy

    Background:

    • Quantitative Phase Imaging (QPI) is a powerful technique for label-free imaging of transparent biological samples.
    • Coherent illumination in QPI can lead to speckle noise, which degrades image quality and resolution.
    • Controlling spatial coherence is crucial for reducing speckle in QPI.

    Purpose of the Study:

    • To investigate a method for speckle reduction in quantitative phase imaging (QPI).
    • To control the spatial coherence of laser illumination using optical diffusers.
    • To improve 3D phase imaging of transparent objects and biological cells.

    Main Methods:

    • Utilized coherent laser illumination with static and moving optical diffusers.
    • Controlled laser spatial coherence by adjusting diffuser particle size and diffusion angle.
    • Employed a common-path QPI configuration for phase mapping.
    • Studied polystyrene micro-beads and MCF-7 breast cancer cells.

    Main Results:

    • Demonstrated speckle reduction in 3D phase imaging through controlled spatial coherence.
    • Successfully mapped phase information of micro-beads and cells with reduced speckle.
    • Observed that tuning diffuser properties effectively controlled coherent speckles.

    Conclusions:

    • The proposed speckle reduction method offers an effective approach for QPI.
    • This technique can enhance lateral resolution in phase images.
    • It provides a way to suppress coherent artifacts, improving the quality of QPI data.