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Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

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Third-harmonic generation Stokes-Mueller polarimetric microscopy.

Lukas Kontenis, Masood Samim, Serguei Krouglov

|August 10, 2017

Summary

This summary is machine-generated.

A new nonlinear Stokes-Mueller polarimetric microscopy method noninvasively characterizes sample ultrastructure. It extracts all nonlinear optical components, revealing complex structures like carrot crystallites with significant chirality.

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Area of Science:

Nonlinear optics
Microscopy
Materials science

Background:

Characterizing material ultrastructure noninvasively is crucial for scientific advancement.
Third-harmonic generation (THG) microscopy offers high resolution but requires advanced techniques for full component analysis.

Purpose of the Study:

To present an experimental implementation of nonlinear Stokes-Mueller polarimetric (NSMP) microscopy in the THG modality.
To demonstrate the technique's capability for comprehensive nonlinear optical property extraction.

Main Methods:

Utilized 64 polarization measurements to extract all eight 2D-accessible nonlinear susceptibility (χ(3)) components.
Applied the NSMP-THG technique to characterize an isotropic glass coverslip and carotenoid crystallites in Daucus carota.

Main Results:

Successfully extracted all eight χ(3) components for the tested samples.
Observed complex χ(3) components in carrot crystallites, indicating a significant chiral contribution.
Demonstrated the noninvasive nature of the technique for ultrastructural characterization.

Conclusions:

The developed NSMP-THG microscopy is a powerful tool for complete nonlinear optical characterization.
The technique enables detailed, noninvasive ultrastructural analysis of materials, including biological samples.
The findings highlight the potential for investigating chiral properties in complex molecular structures.