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Long non-coding RNA expression profile in cervical cancer tissues.
Hua Zhu1,2, Xiangjian Chen3, Yan Hu2
1Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510280, P.R. China.
Oncology Letters
|August 10, 2017
Summary
Researchers identified 3356 differentially expressed long non-coding RNAs (lncRNAs) in cervical cancer (CC) tissues. Two specific lncRNAs showed significant expression changes, suggesting their potential roles in CC development and offering new avenues for research.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Cervical cancer (CC) is a significant health challenge for women globally.
- Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in various cancers, including CC, influencing tumor suppression and oncogenic pathways.
Purpose of the Study:
- To investigate the expression profile of lncRNAs in cervical cancer tissues compared to adjacent non-cancerous tissues.
- To identify differentially expressed lncRNAs associated with cervical cancer for potential diagnostic or therapeutic relevance.
Main Methods:
- Microarray analysis was employed to profile lncRNA and protein-coding mRNA expression.
- Differential expression analysis was performed to compare lncRNA levels between CC and non-cancerous tissues.
- Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was used for validation of selected lncRNAs.
Main Results:
- A total of 3356 lncRNAs exhibited significantly different expression patterns in CC tissues versus adjacent non-cancerous tissues.
- Among these, 1,857 lncRNAs were found to be upregulated in CC tissues.
- Validation using RT-qPCR confirmed significantly different expression for 2 randomly selected lncRNAs.
Conclusions:
- The identified differentially expressed lncRNAs may play crucial roles in the pathogenesis of cervical cancer.
- Expression data provides a foundation for further research into the specific involvement of lncRNAs in CC development.
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