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CMTM5 inhibits renal cancer cell growth through inducing cell-cycle arrest and apoptosis

Bing Cai1,2, Yunbei Xiao2, Yeping Li2

  • 1Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

Oncology Letters
|August 10, 2017
PubMed

Insights

CKLF-like MARVEL transmembrane domain-containing 5 (CMTM5) is downregulated in renal cell carcinoma (RCC). Restoring CMTM5 suppresses RCC cell proliferation and migration, indicating its tumor suppressor role and potential as a therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • CKLF-like MARVEL transmembrane domain-containing 5 (CMTM5) is a potential tumor suppressor in various cancers.
  • The role of CMTM5 in human renal cell carcinoma (RCC) is not well understood.

Purpose of the Study:

  • To investigate the expression pattern of CMTM5 in RCC.
  • To determine the anti-proliferative effects of CMTM5 in RCC cells.

Main Methods:

  • Quantitative reverse transcription-PCR, immunohistochemistry, and western blotting were used to assess CMTM5 expression.
  • ACHN RCC cells were transfected with CMTM5 lentivirus to evaluate cell viability, migration, apoptosis, and cell cycle.
  • Cell Counting kit-8, Transwell assay, and flow cytometry were employed for functional analyses.

Main Results:

  • CMTM5 expression was significantly downregulated in RCC tissues compared to adjacent non-malignant tissues.
  • Enforced CMTM5 expression inhibited ACHN cell proliferation and induced G0/G1 phase arrest.
  • Restoration of CMTM5 expression suppressed RCC cell migration and invasion.

Conclusions:

  • CMTM5 acts as a tumor suppressor in human RCC.
  • CMTM5's ability to suppress RCC cell viability suggests its potential as a therapeutic target for this malignancy.
Keywords:
CMTM5renal cell carcinomatumor suppressor

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