Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

14.7K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
14.7K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

11.6K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
11.6K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.6K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.6K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

21.4K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
21.4K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

921
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
921

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Early Detriment Analysis of First-Line Nivolumab plus Ipilimumab-Based Therapy in Patients with Metastatic Non-Small Cell Lung Cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research·2026
Same author

Chromatix: a differentiable, GPU-accelerated wave-optics library.

Nature methods·2026
Same author

Long-Term Clopidogrel Versus Aspirin Monotherapy After Drug-Eluting Stent Implantation: A Nationwide Real-World Comparative Study.

The American journal of cardiology·2026
Same author

Disease characteristics and treatment outcomes in patients with resected early-stage ALK-positive non-small cell lung cancer from the randomized ALINA trial.

Lung cancer (Amsterdam, Netherlands)·2026
Same author

Chromatix: a differentiable, GPU-accelerated wave-optics library.

bioRxiv : the preprint server for biology·2026
Same author

Water structure and dynamics under distinct microheterogeneity in DMSO-water and acetone-water mixtures.

The Journal of chemical physics·2026
Same journal

Integrated multi-assessment and structural performance index framework for stacking-sequence optimisation of natural fibre reinforced laminates.

Scientific reports·2026
Same journal

SuperiorGAT: graph attention networks for sparse LiDAR point cloud reconstruction in autonomous systems.

Scientific reports·2026
Same journal

The effect of stretching the pectoralis major, sternocleidomastoid, and iliopsoas muscles on 800 m swimming performance in master swimmers.

Scientific reports·2026
Same journal

ISNR-PQC: isometry noise resilience post quantum cryptography primitive.

Scientific reports·2026
Same journal

Identification of high-yielding and stable genotypes of barley in the cold climate of Iran using AMMI and GGE biplot models.

Scientific reports·2026
Same journal

Bayesian negative binomial modelling of spatial and temporal patterns of road traffic deaths in Ghana.

Scientific reports·2026
See all related articles

Related Experiment Video

Updated: Feb 25, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.4K

Smartphone-based multi-contrast microscope using color-multiplexed illumination.

Daeseong Jung1, Jun-Ho Choi2, Soocheol Kim1

  • 1Yonsei University, School of Mechanical Engineering, Seoul, 03722, Republic of Korea.

Scientific Reports
|August 10, 2017
PubMed
Summary
This summary is machine-generated.

We developed a portable smartphone microscope using color-coded light-emitting-diode (LED) microscopy (cLEDscope) to capture bright-field, dark-field, and differential phase contrast images of biological specimens in a single shot.

More Related Videos

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

26.3K
Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

10.3K

Related Experiment Videos

Last Updated: Feb 25, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.4K
A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

26.3K
Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

10.3K

Area of Science:

  • Biomedical Engineering
  • Optical Microscopy
  • Mobile Health Technology

Background:

  • Traditional microscopy requires bulky equipment.
  • Multi-contrast imaging offers richer specimen information.
  • Integrating advanced imaging into portable devices is challenging.

Purpose of the Study:

  • To develop a portable, multi-contrast microscope using smartphone technology.
  • To enable bright-field, dark-field, and differential phase contrast imaging.
  • To create a cost-effective and accessible imaging solution.

Main Methods:

  • Developed a smartphone add-on module with a patterned color micro-LED array and miniature objective.
  • Utilized color-coded light-emitting-diode (LED) microscopy (cLEDscope) for illumination.
  • Implemented an Android app for image acquisition, computation, and real-time display.

Main Results:

  • Achieved single-shot, multi-contrast imaging (bright-field, dark-field, differential phase contrast).
  • Demonstrated the functionality with various thin biological specimens.
  • The add-on module is easily attachable to a smartphone.

Conclusions:

  • The smartphone-based cLEDscope provides a portable and versatile multi-contrast microscopy solution.
  • This technology can enhance point-of-care diagnostics and remote biological analysis.
  • The system offers a low-cost alternative to conventional microscopy.