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Double-stranded RNA induces chicken T-cell lymphoma apoptosis by TRIF and NF-κB

Haitao Zou1,2, Ruixue Su1,2, Jing Ruan1,2

  • 1Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, No. 12 East Wenhui Road, Yangzhou, Jiangsu, 225009, P. R. China.

Scientific Reports
|August 10, 2017
PubMed

Insights

Toll-like receptor-3 (TLR3) activation triggers apoptosis in chicken lymphoma cells. This pathway, independent of interferon, involves TRIF and NF-κB, offering potential for new lymphoma and oncovirus infection treatments.

Area of Science:

  • Immunology
  • Cell Biology
  • Oncology

Background:

  • Toll-like receptor-3 (TLR3) activates immune responses and has anti-tumor effects.
  • Its role in chicken lymphoma is not well understood.

Purpose of the Study:

  • To investigate the effect of TLR3 activation on Marek's disease lymphoma-derived chicken cells (MDCC-MSB1).
  • To elucidate the signaling pathway involved in TLR3-mediated apoptosis in these cells.

Main Methods:

  • Activation of TLR3 using poly (I:C) agonist.
  • Pharmacological approaches to study the signaling pathway.
  • Assessment of cell proliferation and apoptosis.

Main Results:

  • Poly (I:C) activated the TLR3 pathway, inhibiting MDCC-MSB1 cell proliferation.
  • TLR3 activation induced caspase-dependent apoptosis.
  • An interferon-independent mechanism involving TRIF and NF-κB mediated the apoptosis.

Conclusions:

  • TLR3 activation induces apoptosis in chicken T-cell lymphoma via an interferon-independent pathway.
  • This finding is novel for chicken T-cell lymphoma and its signaling pathways.
  • The mechanisms could inform new treatments for lymphomas and oncovirus infections.

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